草海桐叶的主要挥发性化学成分及抑菌活性  

Main Volatile Chemical Constituents and Antimicrobial Activity of Scaevola Sericea Leaves

作　　者：李敏[1] 刘红星[1] 黄初升[1] 陆倩[1] 

机构地区：[1]广西师范学院化学与材料科学学院,广西南宁530001

出　　处：《化工技术与开发》2015年第1期10-14,共5页Technology & Development of Chemical Industry

基　　金：广西教育厅科研资助项目(桂教科研[2010]14号);广西研究生创新计划项目(201210603R03)

摘　　要：对广西半红树植物草海桐叶的石油醚提取样和乙酸乙酯提取样,采用GC-MS技术及琼脂扩散法的牛津杯法,进行化学成分分析和抑菌活性研究,样品中各化学成分的相对含量用面积归一法确定。在实验条件下,草海桐叶的石油醚部分分离出21个峰,鉴定了16个,占总峰面积的90.46%,其主要成分为棕榈酸(27.59%)、亚麻酸(14.15%)、植醇(11.58%)等;该部分对肺炎克伯氏菌的抑菌圈直径达到13mm。乙酸乙酯部分共分离出36个峰,鉴定了29个,占总峰面积的78.15%,其主要成分为别嘌呤醇(7.41%)、吲哚-3-乙酸肼(6.55%)、4,6-二甲基-7-乙基氨基香豆素(4.59%)、棕榈酸(5.2%)、莨菪亭(4.71%)、亚麻酸(6.3%)、7-羟基香豆素(4.72%)等;该部分对普通变形杆菌的抑菌圈直径达到15mm,对金黄色葡萄球菌、肺炎克伯氏菌和铜绿假单胞菌均达到14mm。The main volatile chemical constituents of petroleum ether and ethyl acetate extraction of scaevola sericea leaves were analyzed by gas chromatography-mass spectrometry（GC-MS） and then the antibacterial activity of the extraction was also studied with Oxford-cup agar diffusion method. It was revealed that 21 peaks appeared in GC-MS spectrogram of the petroleum ether sample,of which 16 peaks had been identified ,accounting for 90.46% of the total area. There were n-hexadecanoic acid（27.59%）,9,12,15-octadecatrienoic acid（14.15%）, phytol（11.58%） and so on. The crude sample showed antibacterial activity with zones of inhibition of 13mm against keboshi bacteria pneumonia.besides,36 peaks appeared in GC-MS spectrogram of the ethyl acetate extraction and 29 peaks, 78.15% of the total area, had been identified. There were allopurinol （7.41%）,1H-indole-3-acetic acid, hydrazide （6.55%）, 2H-1-benzopyran-2-one, 7-（ethylamino）-4,6-dimethyl- （4.59%） , n-hexadecanoic acid（5.2%）, scopoletin（4.71%）, 9,12,15-octadecatrienoic acid （6.3%）, 7-hydroxycoumarin （4.72%） and so on. The crude sample showed antibacterial activity with zones of inhibition of 15mm against proteus vulgaris, and 14mm against staphylococcus aureus, keboshi bacteria pneumonia and pseudomonas aeruginosa.

关 键 词：草海桐 化学成分 抑菌活性 

分 类 号：R284.2[医药卫生—中药学]