鼻咽癌组织、细胞及患者外周血E-钙黏蛋白基因启动子的异常甲基化  

Aberrant methylation around E-cadherin gene promoter region in nasopharyngeal carcinoma tissues and cell lines,and the peripheral blood of patients

作　　者：冉永刚[1] 游颜杰[2] 黄继红[3] 李文梅[2] 苑艺[2] 李海军[4] 赵新雷[5] 

机构地区：[1]中国人民解放军白求恩医务士官学校训练部,石家庄050081 [2]漯河医学高等专科学校药学系,河南漯河462000 [3]汕头大学医学院附属肿瘤医院中药房,广东汕头515041 [4]四川省内江市第二人民医院放疗科,641000 [5]中国人民解放军白求恩医务士官学校附属医院信息科,石家庄050000

出　　处：《广东医学》2015年第2期208-211,共4页Guangdong Medical Journal

基　　金：河南省科技发展计划项目(编号:142102310464);漯河医学高等专科学校自然科学项目(编号:2013;Y-J You)

摘　　要：目的探讨鼻咽癌组织和细胞E-钙黏蛋白(E-cadherin)基因表达水平与启动子甲基化的相关性,以及患者外周血E-cadherin甲基化作为肿瘤分子标志物的可能。方法采用RT-PCR检测鼻咽癌组织及两种鼻咽癌细胞株HNE1和CNE2中E-cadherin表达水平,以甲基化特异性PCR及硫化测序方法甲基化状态分析鼻咽癌组织、两种鼻咽癌细胞株及鼻咽癌患者外周血中E-cadherin启动子区域的甲基化状态。结果 40例鼻咽癌组织中E-cadherin表达下调率为80%(32/40),甲基化率为65%(26/40),5例正常鼻咽组织中未检测到E-cadherin甲基化,鼻咽癌患者外周血中E-cadherin甲基化率为45%(9/20)。结论基因启动子区域高甲基化状态可能是导致鼻咽癌组织中E-cadherin表达下调的重要机制,经外周血检测E-cadherin甲基化将为鼻咽癌的筛查诊断提供敏感、特异的无创检测方法。Objective To investigate the expression and methylation status of E - cadherin in nasopharyngeal car- o;noma （NPC）, and to evaluate efficacy of methylated E -cadherin in peripheral blood as a potential biomarker on the di- agnosis of NPC, Methods RT - PCR and methylation - specific PCR （MSP） were performed to analyze the mRNA ex- pression levels and methylation status of E - cadherin in primary NPC specimens and two tumor cell lines. MSP was also used to observe the methylated E - cadherin in primary NPC specimens, two tumor cell lines and peripheral blood samples of NPC patients. Results Down - regulated E - cadherin expression was detected in 32 of 40 （80%） primary tumors as well as two NPC cell lines, when compared with normal tissues. Methylated E - cadherin was observed in 26 of 40 （65%） primary tumors but not in normal tissues. Moreover, E - cadherin methylation was also detected in 9 of 20 （45%） periph- eral blood samples of NPC patients. Conclusion Promoter methylation may be one of the mechanisms inducing E - cad- herin silencing. Detection of methylated E - cadherin in peripheral blood suggests potential clinical application for nonin- vasive diagnosis and disease monitoring of NPC.

关 键 词：鼻咽癌 桥粒钙黏蛋白质类 DNA甲基化 

分 类 号：R739.63[医药卫生—肿瘤]