中草药饲料添加剂对黄鳝性激素含量的影响  

The Influence of Chinese Herbal Medicine Feed Additiveon on Eel (Monopterus albus) Sex Hormone Levels

作　　者：赵庆凯[1,2] 丁炜东[1,2] 邴旭文[1,2] 王建新[2] 

机构地区：[1]南京农业大学无锡渔业学院,江苏无锡214081 [2]中国水产科学研究院淡水渔业研究中心,江苏无锡214081

出　　处：《中国农学通报》2014年第32期38-42,共5页Chinese Agricultural Science Bulletin

基　　金：公益性行业科研专项"鳝鱼产业技术研究与试验示范"(201003076);江苏省自然科学基金项目"建鲤基因组中具有转录活性的反转录转座子ty3-gypsy的分离和转录活性分析"(BK2011184);江苏省科技支撑计划"黄鳝规模化生态繁殖及高效养殖技术的研究与示范"(BE2013315)

摘　　要：试验以初始平均体重为(41.2±2.3)g的雌黄鳝和初始体重为(113.5±5.6)g雄黄鳝为研究对象,分别配置含中草药质量浓度为3%、4%、5%的鱼糜饲料,每组设置3个重复,连续投喂31 d(其中前10 d为无中草药的训食阶段,后21 d为添加中草药试验阶段)并进行抽血采样,测定其血清内雌二醇与睾酮的含量,来评价饲料中的中草药浓度对黄鳝性激素含量的影响,实验结果表明:(1)与对照组相比,摄食不含中草药饲料的亲鱼血液中雌二醇与睾酮的含量显著高于含中草药饲料的亲鱼(P<0.05),并且亲鱼血清内雌二醇与睾酮的含量随着中草药浓度的增加而降低(P<0.05);(2)从时间的角度分析得出的结论是,雌鱼血清内的雌二醇含量与雄鱼血清内的睾酮含量,在各个试验组中,都随着时间的积累而呈现降低的趋势(P<0.05)。The female eel of initial average weight（41.2＋2.3） g and the male eel of initial weight（113.5＋5.6） g were took as the research objects. The test used minced fish feed with concentration of Chinese herbal medicine at the rate of 3%, 4%, 5%, respectively, and set up three repeats for each group. Continuous feeding was conducted for 31d（the first 10 d was the training phase without Chinese herbal medicine, followed by 21 d of trial phase with Chinese herbal medicine added）. Blood sample was obtained to determine the content of serum estradiol and testosterone and evaluate the influence of the concentration of Chinese herbal medicine on sex hormone levels of eel. The experimental results show that：（1） compared with control group, estradiol and testosterone levels in blood of parent fish that fed without Chinese herbal medicine were significantly higher than that fed with Chinese herbal medicine（P〈0.05）, and serum levels of estradiol and testosterone decreased with the increase of concentration of Chinese herbal medicine（P〈0.05）;（2） the serum estradiol level of females and serum testosterone level of males declined with time（P〈0.05） in each test group.

关 键 词：中草药 黄鳝 雌二醇 睾酮 

分 类 号：S961.2[农业科学—水产养殖]