人乳头瘤病毒E6/E7 mRNA在不同程度宫颈病变中的应用价值  

The diagnostic value of human papilloma virus E6/E7 mRNA detection in different degrees of cervical lesions

作　　者：廖焕兰[1] 陈富[1] 王丽娜[1] 周妙姬 冯辉 邱峰[1] 

机构地区：[1]广东省中医院检验科,广州510120 [2]凯普生物科技有限公司,广东潮州521000

出　　处：《检验医学与临床》2014年第23期3258-3259,3262,共3页Laboratory Medicine and Clinic

基　　金：广东省中医药局科研项目(20122126);广东省人口和计划生育委员会科研项目(20133061)

摘　　要：目的探讨人乳头瘤病毒(HPV)E6/E7mRNA水平在不同程度宫颈病变中的应用价值。方法选取2011年4月至2013年4月广东省中医院妇科门诊收治的HPV感染患者430例,将HPV-DNA阳性标本根据病理资料分为炎症组、CINⅠ组、CINⅡ组、CINⅢ组和宫颈癌组。用反转录聚合酶链反应(RT-PCR)检测各组标本的HPV E6/E7mRNA水平,比较不同宫颈病变组间HPV-DNA病毒载量及HPV E6/E7mRNA阳性检出率的差异情况。结果随着宫颈病变程度增加,HPV-DNA病毒载量未呈现逐级递增的趋势,各病变组间的病毒载量两两比较差异无统计学意义(P>0.05);而HPV E6/E7mRNA阳性检出率则逐级递增,除炎症组与CINⅠ组的检出率比较差异无统计学意义(P>0.05)之外,其余各组之间的检出率两两比较差异均有统计学意义(P<0.05)。结论 HPV E6/E7mRNA与宫颈病变程度有高度的相关性,可作为宫颈疾病筛查的重要检测指标。Objective To investigate the diagnostic value of human papilloma virus （HPV） E6/E7 mRNA in different degrees of cervical lesions .Methods A total of 430 patients with HPV infection ,received by the Outpatient Department of the Traditional Chinese Medicine Hospital of Guangdong Province ,were enrolled and divided into in‐flammation ,cervical epithelial atypical hyperplasia（CIN） Ⅰ ,CIN Ⅱ ,CIN Ⅲ and cervical cancer groups according to pathological data .The reverse transcription polymerase chain reaction （RT‐PCR） method was used to detect the HPV‐DNA viral load and HPV E6/E7 mRNA level .And the HPV‐DNA viral load and positive detectable rathe of HPV E6/E7 mRNA were compared among the different degrees of cervical lesions groups .Results The HPV DNA viral load was not increased with the cervical lesion degree ,and there was no significant differences between any two groups（P〉0 .05） .While the positive detectable rate of HPV E6/E7 mRNA was increased with the cervical lesion degree ,and there were significant differences between any two groups（P〈0 .05） ,except the comparation between the inflammation and CIN Ⅰ group .Conclusion The positive detectable rate of HPV E6/E7 mRNA might be highly correlated with the degrees of cervical lesions ,and could be an significant maker for the cervical disease screening .

关 键 词：人乳头瘤病毒 E6基因 E7基因 信使RNA 

分 类 号：R737.33[医药卫生—肿瘤]