胃癌手术前后血循环miR-21表达与胃癌临床病理特征的关系研究  

Relationship between blood circulation miR-21 expression and clinicopathological features of gastric cancer before and after gastric surgery

作　　者：顾荣民[1] 朱明[2] 文旭[1] 陈森清[2] 李金田[2] 马国建[2] 

机构地区：[1]江苏省肿瘤医院普通外科,南京210009 [2]江苏省肿瘤医院遗传与分子生物学研究室,南京210009

出　　处：《国际检验医学杂志》2014年第24期3316-3318,共3页International Journal of Laboratory Medicine

摘　　要：目的探讨胃癌手术前后外周血循环miR-21表达对胃癌临床病理特征的影响。方法 42例原发胃癌手术当日及术后1周抽取外周血,应用实时荧光定量PCR,以U6为内参照基因,检测血浆miR-21相对表达。结果患者胃癌术后血浆miR-21表达与术前相比明显下调(P<0.01),总体相对表达量平均下降了28.2倍;并且发现,有肿瘤家族史的患者术后miR-21表达上调,而无家族史的胃癌患者术后比术前血浆miR-21表达平均下降22倍(P<0.05);实验结果还发现,随着患者分化程度由高向低的变化,患者淋巴结转移率逐步上升,同时术后平均miR-21表达由低向高转变,表现出miR-21相对表达倍数在高、中、中低和低4个分化区上的分布,分别与淋巴结转移率在4个分化区上的分布呈相关性。结论循环外周血miR-21检测对胃癌早期辅助诊断及术后恶性程度判断有临床意义。Objective To investigate effect of peripheral blood miR-21 expression on the clinicopathological features of gastric cancer before and after gastric surgery.Methods The peripheral blood was collected on the operative day and postoperative 7 d in 42 patients with primary gastric cancer surgery.The real-tine quantitative PCR was adopted to detect the relative expression of miR-21 in plasma with U6 as an internal reference gene.Results The plasma miR-21 expression level after gastric cancer operation was significantly down-regulated compared with before operation（P 〈0.01）,the overall relative expression level was dropped by an average of 18.2 times;and it was found that the postoperative miR-21 expression in the patients with the tumor family history was up-regulated,the postoperative plasma miR-21 expression in the patients without the tumor family history was decreased by 22 times compared with before operation（P 〈0.05）;the experimental results also fund that as the patient′s differentiation degree was changed from high to low,the lymph node metastasis rate was gradually increased,while postoperative average miR-21 expression was changed from low to high,showing the relative expression times of miR-21 were distributed in the four differentiated zones of high,middle,middle-low and low,which showed the correlation with the distribution of the lymph node metastasis rate in these four differentiation zones.Conclusion The peripheral blood MiR-21 detection has the clinical significance to early auxiliary diagnosis and the judgement of postoperative malignant degree.

关 键 词：外周血 胃癌 

分 类 号：R735.2[医药卫生—肿瘤]