果王素对肺腺癌小鼠移植瘤生长的抑制作用  

Inhibitory Effects of Kiwi Essence on the Growth of Lung Adencarcinoma in Mice

作　　者：邓湘[1] 贺兼斌[2] 张平[1] 

机构地区：[1]南华大学附属第一医院呼吸内科,湖南衡阳421001 [2]怀化市第一人民医院呼吸内科,湖南怀化418000

出　　处：《临床误诊误治》2014年第12期105-108,共4页Clinical Misdiagnosis & Mistherapy

基　　金：2012年度湖南省卫生厅科研计划(2012-81)

摘　　要：目的观察果王素对肺腺癌小鼠移植瘤生长的抑制作用,并探讨其作用机制。方法接种Lewis肺腺癌细胞构建肺腺癌小鼠移植瘤模型,将40只接种Lewis肺腺癌细胞的C57BL/6J小鼠随机分为对照组(予0.9%氯化钠注射液)、果王素组(予果王素180 mg/kg)、化疗组(予顺铂联合吉西他滨)、低剂量果王素并化疗组(予果王素60 mg/kg联合顺铂、吉西他滨)、高剂量果王素并化疗组(予果王素180 mg/kg联合顺铂、吉西他滨),每组各8只。肿瘤细胞接种4 d成瘤后予相应的药物干预,接种20 d后处死小鼠,剥离皮下肿瘤。应用免疫组织化学法检测移植瘤组织中血管内皮生长因子(VEGF)、Survivin和B细胞淋巴瘤/白血病-2(Bcl-2)的表达水平。结果各药物干预组肿瘤的生长均受到抑制,果王素组、化疗组、低剂量果王素并化疗组、高剂量果王素并化疗组抑瘤率分别为14.95%、41.77%、44.21%、59.68%,组间比较差异均有统计学意义(P<0.05,P<0.01)。各药物干预组VEGF、Survivin、Bcl-2表达均较对照组低,差异均有统计学意义(P<0.05,P<0.01)。结论果王素能抑制小鼠Lewis肺腺癌的生长,且果王素与顺铂、吉西他滨联用的抗肿瘤作用更明显,可能是通过抑制微血管形成及Survivin、Bcl-2表达发挥作用。Objective To observe the inhibitory effect of Kiwi essence on xenograft growth of lung adenocarcinoma in mice. Methods Forty C57BL/6J mice bearing Lewis cells were randomized into five groups： control group （0. 9% sodium chloride） , Kiwi essence group （ Kiwi essence 180 mg/kg） , chemotherapy group （ cisplatin and gemcitabine） , low-dose Kiwi essence combined chemotherapy group （ Kiwi essence 60 mg/kg combined cisplatin and gemcitabine） , high-dose Kiwi essence combined chemotherapy group （180 mg/kg Kiwi essence combined cisplatin and gemcitabine）. 4 days after being inoculated with adenocarcinoma cells, drug intervention was given in five groups of mice. Mice were sacrificed at day 20 after tumor inoc-ulation. The subcutaneous tumors were stripped for histological examination. The tumor inhibitory rate was measured. The ex-pressions of vascular endothelial growth factor （ VEGF） , Bcl-2 and Survivin were determined by immunohistochemistry method with image analyzing system. Results There were significantly lower values in subcutaneous tumor volume and weight in drug-treated groups. The inhibitory rate was 14. 95%, 41. 77%, 44. 21% and 59. 68% in Kiwi essence group, chemotherapy group, low-dose Kiwi essence combined chemotherapy group, high-dose Kiwi essence combined chemotherapy group respec-tively （P〈0. 05, P〈0. 01）. VEGF, Survivin, Bcl-2 in each group were lower than in the control group （P〈0. 05, P〈0. 01）. Conclusion Kiwi essence can produce a synergetic inhibitory activity against tumor growth of lung adenocarcinoma and enhance GP therapeutic regimen （ cisplatin plus gemcitabine） to inhibit tumor growth of lung adenocarcinoma, which may be associated with the effect of suppressing the expression of angiogenesis, Survivin and Bcl-2.

关 键 词：小鼠 肺肿瘤 原癌基因蛋白质C-BCL-2 血管内皮生长因子类 果王素 

分 类 号：R734.2[医药卫生—肿瘤]