VEGF-C上调ROCK-2/Moesin信号通路促进子宫内膜癌转移  

VEGF-C Promotes Endometrial Cancer Cell Invasion Via Activation of ROCK-2/Moesin Cascade

作　　者：程杨[1] 顾正田[1] 

机构地区：[1]广州市第一人民医院妇产科,广东广州510180

出　　处：《湖北民族学院学报（医学版）》2014年第4期5-8,共4页Journal of Hubei Minzu University(Medical Edition)

基　　金：广州市医药卫生科技项目(20131A011015);广州市科技计划项目(2014J4100113)

摘　　要：目的研究VEGF-C(Vascular endothelial growth factor C)促子宫内膜癌细胞侵袭能力的影响,探讨Rho相关蛋白激酶2(Rho-associated kinase 2,ROCK-2)/膜突蛋白(moesin)信号通路在其中的作用。方法体外培养宫颈癌细胞株ishikawa细胞,免疫印迹法测定VEGF-C对ROCK-2/moesin蛋白的表达和磷酸化的调控作用。采用Transwell侵袭小室法观察VEGF-C对Ishikawa细胞侵袭能力的影响。转染moesin siRNA抑制moesin表达后,进一步观察VEGF-C对细胞侵袭的影响。结果 VEGF-C(100!g/L)处理细胞24 h后,可显著增高ROCK-2蛋白、moesin蛋白、磷酸化moesin蛋白的表达。VEGF-C单克隆抗体阻断可上述作用。以ROCK-2特异性抑制剂Y-27632预处理细胞后,可明显抑制VEGF-C促moesin蛋白表达及磷酸化的作用。VEGF-C可明显促进Ishikawa细胞的侵袭能力,与对照组比较,其增加幅度为(286.4±25.2)%(n=5,P<0.01)。而在转染特异性moesin siRNA 48 h抑制moesin表达后,VEGF-C促细胞侵袭的能力明显减弱,抑制率为(75.6±9.8)%(n=5,P<0.01)。结论 VEGF-C可促进子宫内膜癌的侵袭能力,这一作用由ROCK-2/moesin信号通路介导。Objective To investigate the influence of vascular endothelial growth factor C on en-dometrial cancer invasion and to explore the role of Rho-associated kinase 2 （ ROCK-2）/moe-sin cascade in this process.Methods Endometrial cancer cell line Ishikawa cells were cultured. Western blot method was used to detect the modulatory effects of VEGF-C on ROCK-2, moe-sin protein expression and phosphorylation.Transwell invasion chamber assay was performed to access the effect of VEGF-C on cell invasion under the tranfs ection with specific moesin siRNA.Results Treatment with VEGF-C （100μg/L） for 24h enhances the ROCK-2 and moesin expression and phosphorylation, which is inhibited by VEGF-C.monoclonal antibody.Mean-while,the specific inhibitor of ROCK-2,Y-27632,largely inhibits VEGF-C enhanced-moesin ex-pression and phosphorylation.VEGF-C largely promoted Ishikawa cell invasion, with the in-creasing magnitude of （286.4±25.2）%（n=5,P〈0.01）.After Ishikawa cells were transfected with moesin siRNA,the effects of VEGF-C on cell invasion were largely inhibited, with the in-hibitory rate of （75.6±9.8）%（n=5,P〈0.01）.Conclusions VEGF-C promotes endometrial cancer cell invasion, which is mediated by the activation of ROCK-2/moesin cascade.

关 键 词：子宫内膜癌 血管内皮生长因子C 膜突蛋白 侵袭 

分 类 号：R737.33[医药卫生—肿瘤]