HPLC法测定黄牡丹不同部位中没食子酸和丹皮酚的含量  

Determination of Gallic Acid and Paeonol in Different Medicinal Parts of Paeonia delavayi var. lutea by HPLC

作　　者：王琳[1] 周浓[1] 代亨英 

机构地区：[1]大理学院药学院,大理671000

出　　处：《医药导报》2015年第2期228-231,共4页Herald of Medicine

基　　金：国家自然科学基金资助项目(81260622)

摘　　要：目的建立黄牡丹中活性成分没食子酸和丹皮酚的含量测定方法,并考察黄牡丹不同部位中没食子酸和丹皮酚的含量差异。方法采用高效液相色谱（HPLC）法,Agilent TC-C18柱（4.6 mm×150 mm,5μm）;流动相为乙腈-0.1%磷酸溶液,梯度洗脱：0 min→25 min→45 min,乙腈0%→24%→36%,流速为0.8 m L·min-1;检测波长为274 nm;柱温为30℃。结果没食子酸、丹皮酚线性范围分别为0.10～2.00μg（r=0.999 8,n=6）、0.005 2～0.104 0μg（r=1.000 0,n=6）,平均回收率（n=9）分别为102.16%（RSD=2.51%）、97.61%（RSD=1.34%）。没食子酸在不同部位的含量分布为果实〉叶〉皮部〉栓皮〉木部〉茎;丹皮酚在不同部位的含量分布为栓皮〉皮部〉木部〉茎,果实、叶中未检出。结论该方法准确、简捷,为黄牡丹药材提供更合理、可靠的质量控制方法。Objective To establish a method to assay the content and distribution of two active ingredients, gallic acid and paeonol in Paeonia delavayi var. lutea. Methods HPLC was adopted. The chromatograph column was Agilent TC-C18 spin column （4.6 mm× 150mm, 5 μm）, mobile phase was acetonitrile-0. 1% phosphoric acid, and gradient elution was used. E]ution condition was 0 min→25 min-→45 min, the content of acetonitrile was 0%→24%→36% , the flow rate was 0.8 mL· min-1 , detection wavelength was 274 nm, and column temperature was 30 ℃. Results The linear range of gallic acid and paeonol was 0.10-2.00μg （ r = 0. 999 8, n = 6） and 0. 005 2-0. 104 0 μg （ r = 1. 000 0, n = 6） , the average recovery was 102.16% （RSD=2.51%） and 97.61% （RSD= 1.34% ）, respectively. The content order of gallic acid in different parts was fruit〉leaf〉epidermis 〉 phelloderm〉wood〉stem. The content order of paeonol in different parts was phellodem 〉 epidermis 〉 wood〉stem, but paeonol was not detected in fruits and leaves. Conclusion This method is accurate and simple, and can serve as a more reasonable and reliable quality control method for extraction of Paeonia delavayi var. lutea.

关 键 词：黄牡丹 没食子酸 丹皮酚 色谱法 高效液相 

分 类 号：R282.71[医药卫生—中药学] R927.2[医药卫生—中医学]