全粉加工兼早熟菜用马铃薯品种LK99再生体系的建立  

Establishment of Plant Regeneration System of Early Vegetable Potato Variety LK99 with Whole Powder Processing

作　　者：胡霞[1] 

机构地区：[1]甘肃省农业科学院林果花卉研究所,甘肃兰州730070

出　　处：《甘肃农业科技》2015年第1期14-16,共3页Gansu Agricultural Science and Technology

基　　金：国家自然科学基金(31060200);甘肃省自然科学基金项目(145RJZA088);甘肃省农业科学院中青年基金(2014GAAS20)部分内容

摘　　要：以马铃薯品种LK99试管苗茎段为外植体,分别在6种培养基上进行愈伤组织的诱导,比较接种28 d时的出愈率及愈伤组织的形态,并将胚性愈伤接到8种不同分化培养基上进行再生苗的分化。结果表明,在接种28 d后,6种培养基均可诱导出大量的愈伤组织,以MS+2.5 mg/L 6-BA+0.5 mg/L NAA+3 mg/L GA3+0.5 mg/L 2,4-D培养基上胚性愈伤诱导率最高,生长状态最好。胚性愈伤在MS+2.5 mg/L 6-BA+1.0 mg/L ZT+3.5 mg/L GA3培养基上分化率最高,并且分化苗键壮。To establish high efficient regeneration system of potato （Solarium tuberosum L.） variety LK99, stem segments of LK99 tube seedlings are inoculated on 6 kinds of culture media to induce callus. After 4 weeks from inoculation, the frequency of callus induction and callus morphology are compared. The embryonic callus is inoculated on 8 different differentiation media for differentiation of seedlings. The result shows that after 4 weeks from inoculation, a lot of callus are induced from all 6 kinds of media. The frequency of embryonic callus from the medium E （MS＋2.5 mg/L 6-BA＋0.5 mg/L NAA＋3 mg/L GA3＋0.5 mg/L 2,4-D）is highest, and the growth conditions of embryonic callus is best. The frequency of differentiated seedlings from the medium （MS＋2.5 mg/L 6-BA＋I mg/L ZT＋3.5 mg/L GA3 ）is highest, and the differentiation seedlings is stout.

关 键 词：马铃薯 LK99 愈伤组织 再生体系 

分 类 号：S532[农业科学—作物学]