拟南芥核苷磷酸化酶基因的组织表达及其T-DNA插入突变体鉴定  

Tissue Expression of Nucleoside Phosphorylase Genes and Identification of Its T-DNA Insertion Mutants in Arabidopsis

作　　者：徐文晶[1] 程玉祥[1] 

机构地区：[1]东北林业大学林木遗传育种国家重点实验室,黑龙江哈尔滨150040

出　　处：《贵州农业科学》2015年第1期16-19,共4页Guizhou Agricultural Sciences

基　　金：国家863计划项目"林木优质;速生性状调控基因的分离及育种技术研究"(2013AA102702)

摘　　要：为探明植物核苷磷酸化酶家族基因信息及生理功能,采用PCR扩增的方法,研究了模式植物拟南芥核苷磷酸化酶家族基因在不同组织中的表达,并对其核苷磷酸化酶基因T-DNA插入突变体进行鉴定。结果表明:At4g24340和At4g28940基因在根组织中大量转录表达,其他组织均微量检测到转录表达;At4g24350在各个组织中均有少量转录表达;经T-DNA插入结合转录表达鉴定,At4g28940和At4g24350基因为敲除突变体。To explore information and physiological function of nucleoside phosphorylase genes,by using PCR method,the authors examined gene expression of nucleoside phosphorylase in different tissues of model plant arabidopsis,and further screened T-DNA-inserted mutants of these genes.Results：Transcriptional expression of At4g28940 and At4g28940 is high in root tissues,and slight transcript levels are detected in other tissues,while At4g24350 gene expression is low in various tissues.After the identification of T-DNA insertion and transcriptional levels of the genes,the knockout mutants of At 4g28940 and At 4g24350 have been attained in this study.

关 键 词：拟南芥 核苷磷酸化酶 突变体 

分 类 号：S503.52[农业科学—作物学]