复合建模因素诱导脾虚水湿不化模型大鼠免疫功能的改变  

Changes in Immune Functions of Rat Models with Dampness Stagnancy due to Spleen Deficiency Induced by Compound Molding Factors

作　　者：高洁[1] 韩旭[1] 韩冰冰[1] 崔宁[1] 赵文晓[1] 王世军[1] 

机构地区：[1]山东中医药大学,山东济南250355

出　　处：《中医药信息》2015年第1期40-42,共3页Information on Traditional Chinese Medicine

基　　金：国家重点基础研究发展计划(973计划)资助项目(No.2013CB531803)

摘　　要：目的:研究脾虚水湿不化模型大鼠免疫功能方面的改变。方法:将40只大鼠随机分为空白对照组和模型组,每组20只。空白对照组大鼠给予纯化饲料喂养;模型组大鼠给予高脂低蛋白饲料喂养,同时负重力竭游泳,连续喂养60天。观察大鼠的一般状况,测定大鼠胸腺和脾的脏器指数,检测大鼠血清细胞因子IL-1,IL-2,血清补体C3,Ig G,SIg A的含量。结果:与空白对照组相比,模型组大鼠胸腺的脏器指数,血清细胞因子IL-1,IL-2,血清补体C3,Ig G,SIg A的含量显著降低(P<0.01);脾的脏器指数也出现降低(P<0.05)。结论:脾虚水湿不化模型大鼠会出现免疫功能的降低。Objective： To explore changes in immune functions of rat models with dampness stagnancy due to spleen deficiency. Methods： Divide 40 rats into a control group and a model group, each with 20 rats; rats in the control group were given purification feed; while rats in the model group were given high -fat and low- protein feed, and daily loaded swimming until they were exhausted, for totally 60 days. Observe rats＇ condi- tions and determine the organ index of the thymus and spleen. Test the content of serum cytokines IL - 1, IL- 2 and complement C3, IgG, SIgA. Results： Compared with the control group, the organ index of thy- mus, the content of serum cytokines IL - 1, IL - 2 and complement C3, IgG, SIgA in the model group tended to decrease significantly （P 〈 0. 01 ） ; the organ index of spleen also tended to decrease （ P 〈 0.05 ）. Conclu- sion： Rat models with dampness stagnancy due to spleen deficiency have the decrease of immune functions.

关 键 词：脾虚 水湿不化 大鼠 免疫功能 

分 类 号：R-332[医药卫生]