PEDF对肺鳞状细胞癌SK-MES-1细胞增殖、侵袭和凋亡的影响  

Effects of PEDF on proliferation,invasion and apoptosis of lung squamous cell carcinoma SK-MES-1 cells

作　　者：马建梅[1] 陈承[2] 李雪萍[1] 

机构地区：[1]西安医学院临床医学院临床技能学教研室,陕西西安710021 [2]陕西省西安市第一医院眼科,陕西西安710002

出　　处：《吉林大学学报（医学版）》2015年第1期6-9,I0001,共5页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金资助课题(81100665)

摘　　要：目的:探讨色素上皮衍生因子(PEDF)对肺鳞状细胞癌(鳞癌)SK-MES-1细胞增殖、凋亡和侵袭的影响,阐明PEDF与肺鳞癌发生发展的关系。方法:培养SK-MES-1细胞,设不加PEDF的细胞为阴性对照组,PEDF干预组细胞分别加入180、360和720nmol·L-1 PEDF;MTT法检测各组SK-MES-1细胞增殖抑制率,细胞侵袭实验测定阴性对照组和720nmol·L-1 PEDF干预组SK-MES-1细胞穿透数,倒置显微镜观察各组SK-MES-1细胞形态学,流式细胞术检测各组SK-MES-1细胞凋亡率。结果:PEDF干预组细胞增殖抑制率均显著高于阴性对照组(P<0.01),不同剂量PEDF干预组间比较差异也有统计学意义(P<0.05)。阴性对照组细胞贴壁生长良好,PEDF干预组细胞排列逐渐稀疏,形态改变明显。720nmol·L-1 PEDF干预组SK-MES-1细胞穿透数(31.6±15.8)低于阴性对照组(75.4±19.3),差异有统计学意义(P<0.01)。随着PEDF干预剂量增加,细胞凋亡率逐渐升高,不同剂量PEDF干预组与阴性对照组比较差异均有统计学意义(P<0.05)。结论:PEDF抑制SK-MES-1细胞增殖和侵袭,促进细胞凋亡,对肺鳞癌生长和转移发挥对抗效应。提示PEDF可能成为肺鳞癌患者预后判定、抗癌治疗的有效因子。Objective To explore the effects of pigment epithelium derived factor（PEDF）on the proliferation,apoptosis and invasion of lung squamous cell carcinoma SK-MES-1cells,and to interpret the relationships between PEDF and the carcinogenesis and progression of lung squamous cell carcinoma.Methods The SK-MES-1cells were primarily cultured.The cells without PEDF were usded as negative control group,the cells in PEDF intervention groups were treated with 180,360,and 720nmol·L^-1PEDF.The inhibitory rates of proliferation of SK-MES-1cells were observed by MTT assay,the number of SK-MES-1cells penetrating the membrane in negative control group and 720nmol·L^-1PEDF group were determined by cell invasion experiment,the morphology of SK-MES-1cells was observed by inverted microscope,and the apoptotic rates were detected by flow cytometry.Results The inhibitory rates of proliferation of SK-MES-1cells in PEDF intervention groups were higer than that in negative control group（P〈0.01）,and there were statistics differences among the PEDF groups（P〈0.05）.The cells in negative control group grew well,but the morphological changes of the cells in intervention groups were obvious.The number of the cells penetrating the membrane in 720nmol·L^-1PEDF intervention group（31.6±15.8）was lower than that in negative control group（75.4±19.3）（P〈0.01）.The apoptotic rates of SK-MES-1cells were increased with the increasing of PEDF doses,and there were significant differences between different doses of PEDF intervention groups and negative control group（P〈0.05）.Conclusion PEDF inhibits the proliferation or invasion and promotes the apoptosis of SK-MES-1cells,and play an effect of inhibiting the growth and metastasis of lung squamous cell carcinoma.PEDF may be a potent factor for the prognosis and treatment of lung squamous cell carcinoma.

关 键 词：色素上皮衍生因子 肺肿瘤 SK-MES-1细胞 细胞增殖 侵袭 细胞凋亡 

分 类 号：R734.2[医药卫生—肿瘤]