17-β雌二醇对压力诱导的视网膜神经节细胞凋亡的保护作用及其机制  

Protective effects of 17-β estradiol on apoptosis of retinal ganglion cells induced by pressure and its mechanism

作　　者：王英[1] 许文晶 高志卓 胡萍[1] 杨海燕[1] 饶维[1] 徐韶琳[1] 

机构地区：[1]吉林大学第二医院眼科,吉林长春130041 [2]吉化集团总医院眼科,吉林吉林132013

出　　处：《吉林大学学报（医学版）》2015年第1期60-63,I0002,共5页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅科研基金资助课题(20090460);吉林大学白求恩医学科研支持计划项目资助课题(2013206043)

摘　　要：目的:探讨17-β雌二醇(E2)对压力诱导的视网膜神经节细胞(RGC-5细胞)凋亡的保护作用,并阐明其可能作用机制。方法:RGC-5细胞分为正常对照组、单纯加压组和加压联合E2组。取处于对数生长期细胞,应用MTT法检测各组细胞相对存活率;Hoe33342核染色法检测各组细胞凋亡形态;Western blotting法检测各组细胞凋亡相关蛋白bax、bcl-2、P53、iASPP和cleaved-caspase-3表达水平。结果:MTT法检测,与对照组比较,单纯加压组及加压联合E2组细胞存活率均降低(P<0.05);加压联合E2组细胞存活率高于单纯加压组(P<0.05)。细胞染色检测,加压联合E2组细胞核浓缩及凋亡小体出现率低于加压组。与对照组比较,单纯加压组及加压联合E2组bax、P53和cleaved-caspase3表达水平均升高(P<0.05),bcl-2和iASPP表达水平均降低(P<0.05);与单纯加压组比较,加压联合E2组bax、P53和cleaved-caspase-3表达水平降低(P<0.05),bcl-2和iASPP表达水平升高(P<0.05)。结论:E2对加压条件下RGC-5细胞有保护作用,可以减少加压条件下的RGC-5细胞的凋亡。Objective To explore the protective effects of 17-βestradiol（E2）on the apoptosis of cultured retinal ganglion cells（RGC-5cells）induced by pressure,and to clarify its mechanism.Methods The RGC-5cells were divided into control group,80 mmHg pressure group and 80 mmHg pressure combined with E2 group.The cells at logarithmic growth phase were used in the experiment.The cell viabilities in various groups were determined by MTT assay.The apoptotic morphology of the cells in various groups was determined by Hoe33342 staining.The expression levels of bax,bcl-2,P53,iASPP,and cleaved-caspase-3were determined by Western blotting method.Results The MTT assay showed that compared with control group,the viabilities of the RGC-5cells in pressure group and pressure combined with E2 group were decreased（P 0.05）;compared with pressure group,the viability of the RGC-5cells in pressure combined with E2 was increased（P〈0.05）.The results of cell staining showed that compared with pressure group,the chromatin condensation and formation of apoptotic bodies in the RGC-5cells in pressure combined with E2 group were decreased.The Western blotting results showed that compared with control group,the expression levels of bax,P53 and cleaved-caspase-3were increased（P〈0.05）,the expression levels of bcl-2and iASPP were decreased（P〈0.05）in the RGC-5cells in pressure group and pressure combined with E2group;compared with pressure group,the expression levels of bax,P53 and cleavedcaspase-3were decreased（P〈0.05）,and the expression levels of bcl-2and iASPP were increased（P〈0.05）in the RGC-5cells in pressure combined with E2 group.Conclusion E2 has the protective effects on the RGC-5cells and could decrease the apoptosis of RGC-5cells under pressure loading condition.

关 键 词：17-Β雌二醇 视网膜神经节细胞 压力 细胞凋亡 

分 类 号：R774.6[医药卫生—眼科]