结缔组织生长因子盖髓剂对牙髓组织形态学的影响及其意义  

Influence of capping agent CCN2 in morphology of dental pulp tissue

作　　者：李雯[1] 尹硕[2] 张娟[1] 王贺[1] 甘抗 马珊珊[4] 张颖丽[1] 

机构地区：[1]吉林大学口腔医院牙体牙髓病科,吉林长春130021 [2]吉林大学口腔医院修复科,吉林长春130021 [3]吉林大学口腔医院综合科,吉林长春130021 [4]吉林大学口腔医院种植科,吉林长春130021

出　　处：《吉林大学学报（医学版）》2015年第1期110-113,I0003,共5页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅科技发展计划项目资助课题(20130102096JC);吉林省长春市科技局科技计划项目资助课题(12SF88)

摘　　要：目的:研究大鼠牙髓机械损伤经结缔组织生长因子(CTGF/CCN2)覆盖于损伤牙髓处后不同时间点大鼠牙髓的组织形态学变化,阐明其作用机制。方法:选择20只Wistar大鼠,平均分为5组,随机选取一组为正常组(不做任何处理),其余4组(3、7、14和28d组)每只大鼠在双侧上颌第一磨牙备洞,左侧为CCN2盖髓剂的实验组,右侧为PBS对照组。分别于3、7、14和28d处死大鼠并取其牙齿标本,经固定、脱钙、切片和HE染色,观察各标本中牙髓组织的形态学变化。结果:对照组大鼠3和7d时穿髓孔下方炎症较实验组明显;14d时炎症反应减轻,有散在的修复性牙本质形成;28d时形成坏死病灶。实验组大鼠3d时穿髓孔下方聚集大量炎细胞,血管扩张明显;7d时炎症减轻,淋巴细胞相对于对照组少;14d时已无明显炎细胞存在,并形成连续完整的钙化桥;28d时形成大量修复性牙本质。结论:牙髓组织损伤后,CCN2参与了组织结构的重建,提示CCN2是一种良好的盖髓剂。Objective To observe the histomorphological changes of dental pulp of the rats with mechanical injury at different time points after treated with connective tissue growth factor（CTGF/CCN2）and to clarify its mechanism.Methods Tweenty Wistar rats were divided into five groups,one group was randomly selected as normal group,each Wistar rat in the other four groups（groups of 3d,7d,14 dand 28d）was prepared with a cavity on the occlusal surface of maxillary first molars on both sides.CCN2 was applied on the left exposed pulp for the PBS experimental group,and PBS was applied on the right exposed pulp for the control group.Four rats,one in each group,were put to death respectively on the 3rd,7th,14 th and 28 th day after operation.The teeth of each rat were fixed,decalcified and made to sections,which were stained with HE.The histomorphological changes of dental pulp tissue in each sample were observed.Results In control group,the inflammation under the amputated site was more obvious than the experimental group at 3dand 7d;the inflammatory cells were decreased and scattered reparative dentin was observed in 14d;necrosis was observed at 28 d.While in experimental group a large number of inflammatory cells gathered under the amputated site at 3d,as well as the dilatation of blood vessels was observed;7dafter injury the inflammation in experimental group was reduced.Compared with control group,the leukomonocytes were less.After injury for 14 dthe calcified bridge was observed and the inflammatory cells disappeared;28 d after injury the reparative dentin was observed.Conclusion CCN2 participates in the reconstruction of the tissue structure after dental pulp injury.It indicates that CCN2 is a good pulp capping agent.

关 键 词：结缔组织生长因子 牙髓 牙髓复盖术 重建 

分 类 号：R781.05[医药卫生—口腔医学]