绞股蓝大孔树脂分离物对DPPH自由基的清除作用研究  

Scavenging Effect of Macroporous Resin Isolate of Gynostemma Pentaphyllum on Free Radical DPPH

作　　者：肖凡[1] 杨艳芳[1,2] 陈会玲[1] 吴和珍[1,2] 

机构地区：[1]湖北中医药大学中药资源与中药复方教育部重点实验室,武汉430065 [2]湖北中医药大学药学院

出　　处：《中国药师》2015年第2期241-243,共3页China Pharmacist

摘　　要：目的:研究绞股蓝大孔树脂分离物对二苯基苦基苯肼(DPPH)自由基的清除作用。方法:将绞股蓝水煮醇沉的提取物用大孔树脂分离,依次以H2O,40%乙醇,80%乙醇洗脱,得到相应的分离物;采用DPPH自由基体系法测定其抗氧化活性,并与没食子酸、抗坏血酸进行比较。同时,测定各分离物中总皂苷的相对含量。结果:绞股蓝80%乙醇,40%乙醇,H2O分离产物对DPPH自由基的半数抑制浓度分别为0.081 2,0.028 7,0.541 0g·L-1。40%乙醇分离物中皂苷的相对含量最高。结论:绞股蓝不同溶剂大孔树脂分离物均有抗氧化活性,其中40%乙醇分离物的抗氧化性最强。预示绞股蓝皂苷是降脂、降糖以及清除自由基的可能活性物质。Objective： To study the scavenging activity of macroporous resin isolate of gynostemma pentaphyllum on free radical DPPH. Methods： The extract of gynostemma pentaphyllum via water boiling and precipitation with ethanol was separated by macroporous resin respectively eluted by water,40% ethanol and 80% ethanol to obtain the corresponding isolates. The antioxidant activity of the three isolates was determined by free radical DPPH systematic method,and the results were compared with that of gallic acid and ascorbic acid. Results： The 50% inhibitory concentration on free radical DPPH of the three isolates was 0. 081 2,0. 028 7 and0. 541 0 g·L- 1,respectively. The 40% ethanol isolate had the highest saponins concentration. Conclusion： The macroporous resin isolate with different solvent all shows antioxidant activity and the 40% ethanol isolate has the strongest effect,suggesting saponins may be the active substances for the lipid-lowering,hypoglycemic and free radical scavenging activities.

关 键 词：绞股蓝 大孔吸附树脂 二苯基苦基苯肼 抗氧化性 

分 类 号：R285.5[医药卫生—中药学]