一株β-溶血菌的鉴定  

Identification of β-Hemolytic Bacteria

作　　者：徐宏伟[1] 李慧[2,3] 张丽娜[2,3] 李莲瑞[2] 

机构地区：[1]塔里木大学生命科学学院,新疆阿拉尔843300 [2]塔里木大学畜牧科技兵团重点实验室,新疆阿拉尔843300 [3]塔里木大学动物科技学院,新疆阿拉尔843300

出　　处：《西北农业学报》2015年第2期11-15,共5页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家自然科学基金(30960277);新疆生产建设兵团博士基金(2009JC18)

摘　　要：旨在通过细菌16SrRNA鉴定分离得到的一株β-溶血细菌(B1)。提取菌株B1的基因组DNA,利用细菌16SrRNA的通用引物扩增B1菌株的16SrRNA,测序并对序列进行比对分析。结果成功扩增出B1菌株的16SrRNA,条带很亮且无杂带,阴性对照无条带,条带大小为1 499bp,符合16SrRNA 1 500bp的要求;利用NCBI数据库的Blast工具进行核酸比对,结果显示B1菌株与Aneurinibacillus migulanus菌的16S rRNA序列的一致性在99%以上,通过进化树分析可知,B1与Aneurinibacillus migulanus strain A72,Aneurinibacillus migulanus strain ATCC 9999这2种菌株的亲缘关系最近,可以确定B1菌株为短芽孢菌属的Aneurinibacillus migulanus。The purpose of the study is to identify a bacterium B1 producingβ-hemolysis by 16S rRNA isolated in laboratory. Extracted genomic DNA of strain B1, amplified 16S rRNA by using bacteria 16S rRNA universal primers, and the genomic DNA as template, it was sequenced and processed alignment analysis. Successfully amplified the 16S rRNA and the strips were very bright and negative, on the contrary, the control had no strips, the size was 1 499 bp, in line with 16S rRNA 1 500 bp length; using the NCBI database tools for nucleic acid alignments, the results showed that the consis- tency of 16S rRNA sequence was more than 99% between Blstrain and Aneurinibacillus migulanus, the analysis showed that B1 and Aneurinibacillus migulanus strain A72 Aneurinibacillus migulanus strain ATCC 9999 had the closest relationship by phylogenetic.

关 键 词：细菌鉴定 16S RRNA β-溶血 

分 类 号：Q939[生物学—微生物学]