汉黄芩素对卵巢癌HO-8910细胞增殖及凋亡的影响  

Effect of wogonin on the proliferation and apoptosis of ovarian carcinoma HO-8910 cells

作　　者：陈丽君 王娟[2] 李佳[3] 王建[3] 

机构地区：[1]陕西省渭南市富平县医院妇产科,陕西富平711700 [2]西安交通大学医学院第一附属医院妇产科,陕西西安710004 [3]第四军医大学西京医院妇产科,陕西西安710032

出　　处：《中国医药导报》2015年第5期4-7,17,共5页China Medical Herald

基　　金：国家自然科学基金项目(编号81172458/H1621)

摘　　要：目的探讨汉黄芩素对人卵巢癌HO-8910细胞增殖、凋亡的影响,并分析可能的作用机制。方法采用不同浓度(0、20、50、80、100、130μg/m L)的汉黄芩素处理人卵巢癌HO-8910细胞,48 h后分别用MTT法检测细胞活力的变化,AnnexinⅤ/PI双染法检测细胞凋亡的变化,Western blotting分析p21、cyclin B1、p53和Bax蛋白的表达水平。结果随着处理浓度的增加,汉黄芩素剂量依赖性地抑制人卵巢癌HO-8910细胞的增殖(P<0.05),同时诱导细胞大量凋亡。进一步分析证实,汉黄芩素能够剂量依赖性地下调细胞周期相关蛋白cyclin B1的表达,同时上调p21的表达(P<0.05),提示汉黄芩素可通过抑制细胞有丝分裂进程来抑制细胞的增殖。此外,汉黄芩素还能显著诱导p53通路的活化及其下游促凋亡分子Bax的表达(P<0.05);而用p53通路抑制剂PFT-α预处理能够显著性降低汉黄芩素诱导的促卵巢癌细胞的凋亡效应(P<0.05)。结论汉黄芩素能通过抑制细胞有丝分裂进程及调控p53促凋亡通路的活化来抑制卵巢癌细胞的增殖,同时显著诱导细胞大量凋亡,提示汉黄芩素可能具有一定的抗卵巢癌活性,从而为进一步探讨其作为临床治疗卵巢癌的潜在药物奠定理论基础。Objective To explore the role and underlying molecular mechanism of wogonin on the proliferation and apoptosis of ovarian carcinoma HO-8910 cells. Methods Following the stimulation with various doses of wogonin （0, 20, 50, 80, 100, 130 μg/mL） for 48 h, MTY colorimetry was used to detect cell proliferation rate. Cell apoptosis was analyzed using double staining with Annexin V and propidium iodide （Annexin V/PI）. The expression levels of p21, cyclin B1, p53 and Bax were assessed by Western blotting. Results Wogonin dampened ovarian carcinoma HO-8910 cell proliferation and promoted the apoptosis rates in a dose-dependent manner （P 〈 0.05）. Moreover, wogonin dose- dependently down-regulated the expression levels of cell cycle protein cyclin B1, as well as the up-regulation of p21 expression levels （P 〈 0.05）, suggesting that wogonin might inhibit cell growth by suppressing cell cycle. Additionally, an obvious increase in p53 and its downstream Bax was observed in HO-8910 cell stimulated with wogonin statistically （P 〈 0.05）. However, this up-regulation was dramatically attenuated when preconditioning with p53 inhibitor PFT-a （P 〈 0.05）. Conclusion Wogonin can abrogate HO-8910 cell proliferation and enhance cell apoptosis by regulating cell mi-tosis and apoptosis-related p53 pathway, implying as potential suppressor for ovarian cancer. Therefore, this study will support its potential targets for further de- velopment of anti-ovarian cancer therapy.

关 键 词：卵巢癌 汉黄芩素 增殖 凋亡 细胞周期 

分 类 号：R737.31[医药卫生—肿瘤]