Er:YAG激光对人牙周膜细胞增殖和迁移的影响  

Effect of Er:YAG laser irradiation on the cell proliferation and migration of human periodontal ligament cells

作　　者：程群[1] 杨明华[1] 陈斌[1] 刘娟[1] 闫福华[1] 

机构地区：[1]南京大学医学院附属口腔医院牙周病科,南京210008

出　　处：《国际口腔医学杂志》2015年第2期135-139,共5页International Journal of Stomatology

基　　金：南京市科技发展项目(201207002);南京市国际科技合作项目(201303051);江苏省临床医学科技专项项目(BL2013002)

摘　　要：目的比较不同输出功率和照射时间的Er：YAG激光照射对体外培养的人牙周膜细胞增殖、克隆形成、迁移及转化生长因子-β1（TGF-β1）分泌的影响。方法酶消化法培养人牙周膜细胞,传至3~6代用于实验,按不同的输出功率、相同照射时间（0、0.45、0.60、0.75 W,10 s）及不同照射时间、相同输出功率（0、10、30、60 s,0.60 W）对接种细胞行Er：YAG激光照射,通过噻唑蓝法、克隆形成、划痕实验、酶联免疫吸附法观察激光照射对人牙周膜细胞增殖、克隆形成能力、细胞迁移及TGF-β1分泌的影响。结果输出功率为0.45、0.60 W,照射时间10 s时,可促进细胞的增殖及克隆形成（P〈0.05）,迁移速度和TGF-β1分泌也较对照组快,但此两组间差异无统计学意义。照射时间10 s,输出功率0.60 W时,细胞增殖及克隆形成率明显高于对照组（P〈0.05）,迁移速度和TGF-β1分泌较对照组快;60 s反之。结论在适度的功率和照射时间,Er：YAG激光对人牙周膜细胞增殖、克隆形成、迁移及TGF-β1分泌具有促进作用。Objective To compare the effects of different output power and irradiation times of Er：YAG laser irradiation on cell proliferation, clone formation, migration, and transforming growth factor（TGF）-β1 secretion of human periodontal ligament cells. Methods Human periodontal ligament cells were obtained by enzyme digestion, and the cells at the third to the sixth passages were transferred to a tissue culture flask for the experiments. The cells were irradiated by Er： YAG laser at different output powers and same irradiation time（0, 0.45, 0.60, and 0.75 W, at 10 s） as well as at different irradiation times and same power output（0, 10, 30, and 60 s, at 0.60 W）. Methyltetrazolium assay, clone formation assay, wound scratch assay, and enzyme-linked immunosorbent assay were performed to observe the effects of Er：YAG laser on the proliferation, clone formation, migration, and TGF-β1 secretion of human periodontal ligament cells. Results The output powers of 0.45 and 0.60 W can promote cell proliferation, clone formation, migration, and TGF-β1 secretion when the irradiation time is set to 10 s. No significant statistical differences were found between these output powers. In different irradiation time groups, we can obtain better cell proliferation, clone formation, migration, and TGF-β1 secretion results when the irradiation time is set to 10 s. However, we obtain the opposite results when the irradiation time is set to 60 s. Conclusion An appropriate amount of Er：YAG laser irradiation can promote the proliferation, clone formation, migration, and TGF-β l secretion of human periodontal ligament cells.

关 键 词：ER:YAG激光 人牙周膜细胞 细胞增殖 细胞迁移 克隆形成 转化生长因子-Β1 

分 类 号：R781.4[医药卫生—口腔医学]