槟榔碱诱导上皮细胞基质金属蛋白酶9表达上调的分子机制  

Arecoline upregulated matrix metalloproteinase 9 expression in HaCaT cells

作　　者：李明[1,2] 彭解英[2] 吴颖芳[2] 张睿 

机构地区：[1]长沙市口腔医院,长沙410005 [2]中南大学湘雅医院口腔中心,长沙410078

出　　处：《国际口腔医学杂志》2015年第2期166-169,共4页International Journal of Stomatology

基　　金：国家自然科学基金(81371690);湖南省科技厅国际合作计划基金(2012WK4005;2013FJ6009);长沙市科技计划基金(K1-403054-31)

摘　　要：目的探讨槟榔碱诱导上皮细胞HaCaT基质金属蛋白酶9(MMP9)表达的分子机制。方法槟榔碱处理上皮细胞HaCaT,Western blot检测MMP9、核因子(NF)-KB、激活蛋白(AP)-1和Ets-1蛋白的表达,RNAi实验初步确定槟榔碱诱导下调控MMP9表达的转录因子。结果槟榔碱呈时间和剂量依赖性上调上皮细胞HaCaT MMP9的表达,并促进转录因子NF-κB、AP-1和Ets-1的表达。siRNA下调NF-κB表达后,MMP9的表达明显受到抑制,而siRNA下调Ets-1和AP-1的表达后,MMP9的表达下调但不明显。结论槟榔碱通过上调上皮细胞HaCaT NF-κB、AP-1和Ets-1等转录因子的表达促进MMP9的表达上调。初步确定NF-κB是槟榔碱诱导下促进MMP9表达上调的主要转录因子。Objective This study was designed to investigate the mechanism of arecoline-induced matrix metalloproteinase 9（MMP9） expression in epithelial HaCaT cells. Methods HaCaT cells were treated with arecoline having different concentrations at different times. MMP9, nuclear factor（NF）-κB, activator protein（AP）-1, and Ets-1 protein expressions were detected by western blot analysis. Transcription factors of regulated MMP-9 expression induced by arecoline were detected by RNA interference test. Results Arecoline upregulated MMP9 expression in HaCatcells through the modes of dosage and time symbiosis. Furthermore, arecoline upregulated NF-κB, AP-1, and Ets-1 protein expressions. As detectedby the siRNA test, arecoline affected MMP9 through transcription factors, such as NF-κB, AP-1, and Ets-1, especially NF-κB..Conclusion Arecoline can upregulate MMP9 expression by promoting NF-κB, AP-1, and Ets-1 expressions.and primarily through NF-κB transcription.

关 键 词：槟榔碱 上皮细胞 基质金属蛋白酶9 

分 类 号：R739.8[医药卫生—肿瘤]