姜黄素+5-FU对粘液表皮样癌MEC-1细胞抑制作用的研究  

Study on the Inhibitory Effect of Curcumin and 5-FU on MEC-1 Cells of Mucoepidermoid Carcinoma

作　　者：朱雅文[1] 吴发印[1] 樊治英[1] 

机构地区：[1]遵义医学院第五附属医院

出　　处：《按摩与康复医学》2015年第3期122-124,共3页Chinese Manipulation and Rehabilitation Medicine

基　　金：广东省珠海市卫生局项目资助,编号:2013066

摘　　要：目的：探讨姜黄素＋5-FU对粘液表皮样癌MEC-1的抑制作用。方法：复苏MEC-1细胞株，进行传代培养，当细胞达到对数生长期时进行分组：实验组为姜黄素组、5-FU组、姜黄素＋5-FU组；对照组为PBS组；在MTT实验中，实验组、对照组均由低到高分别设6种不同浓度：5μmol/L、10μmol/L、20μmol/L、40μmol/L、80μmol/L、160μmol/L，观察其对MEC-1细胞株的体外生长活性的影响；在流式细胞术分析实验中，实验组、对照组分别设3种适中浓度：20μmol/L、40μmol/L、80μmol/L，观察其对肿瘤细胞的细胞周期及凋亡率的影响。结果：MTT法观察到6种不同浓度的实验组对肿瘤细胞MEC-1均有明显抑制作用，且这种抑制作用与浓度和作用时间成正相关；流式细胞术分析结果表明，对于MEC-1的细胞周期及凋亡率，3种不同浓度的实验组均能产生影响，且随着浓度的递增，出现G0/G1期随之下降，S期无明显变化，G2/M期细胞随之上升的表现；细胞凋亡率变化表明，姜黄素＋5-FU比单独组更能有效诱导肿瘤细胞MEC-1凋亡。结论：姜黄素与5-FU均能有效抑制粘液表皮样癌MEC-1的生长并使其凋亡，且姜黄素能增强5-FU对MEC-1细胞的抑制效果。Objective：To investigate the inhibitory effect of curcumin and 5-FU on MEC-1 cells of mucoepidermoid carcinoma. Methods： MEC-1 cells were revived and cultured, cells reached a logarithmic growth phase were divided in experimental group, including curcumin group, 5-FU group, curcumin and 5-FU group, and control group which is PBS group; experimental group and control group were arranged concentration of 5μmol/L, 10μmol/L, 20μmol/L, 40μmol/L, 80μmol/L, 160μmol/L from low to high in the MTT experiment, to observe the effects on vitro growth ac-tivity of MEC-1 cells; in the flow cytometry analysis experiment, experimental group and control group were arranged moderate concentration of 20μmol/L, 40μmol/L, 80μmol/L, to observe the effects on cell cycle and apoptosis rate of tumor cells. Results：6 different concentration of experi-mental groups had obviously inhibitory effects on tumor cells and positive correlation with concentration and action time in MTT experiment;flow cytometry analysis showed that 3 different concentration of experimental groups had effects on cell cycle and apoptosis rate of MEC-1,and with the concentration increasing, G0/G1 phase decreased, S phase without significant changes, G2/M phase cells increased;apoptosis rate changes indicated that curcumin and 5-FU could induce the apoptosis of MEC-1 tumor cells more effectively than single group. Conclusion：Curcumin and 5-FU can ef-fectively inhibit the growth of mucoepidermoid carcinoma MEC-1 and make them apoptosis, and curcumin can enhance the inhibitory effect of 5-FU on MEC-1 cells.

关 键 词：粘液表皮样癌 姜黄素 MEC-1细胞 抑制 

分 类 号：R739.8[医药卫生—肿瘤]