透骨消痛颗粒干预OA早期COX-2和iNOS表达的实验研究  

The Experimental Research about granula of penetrating bone removeing pain intervene the expression of COX-2 and iNOS in early stage of osteoarthritis

作　　者：高雪伟 

机构地区：[1]中船武汉六七二医院骨科,湖北武汉430079

出　　处：《转化医学电子杂志》2015年第1期15-17,19,共4页E-Journal of Translational Medicine

摘　　要：目的:通过动物试验探讨透骨消痛颗粒对骨性关节炎早期环氧化酶-2(COX-2)和诱导性一氧化氮合酶(iNOS)表达的影响,研究透骨消痛颗粒在膝骨性关节炎治疗中的相关作用机制.方法:新西兰大白兔采用改良Hulth法造模,造模成功后随机分成3组:模型组、对照组、实验组,和正常组共4组,分别灌胃给药4周后处死动物取材,并观测如下指标:关节腔大体观察,滑膜与软骨组织学观察;关节液内NO、NOS、PGE2含量检测,滑膜软骨中iNOS、COX-2含量检测.结果:4周后关节液中NO、NOS含量、PGE2浓度,滑膜和软骨中iNOS、COX-2含量,模型组、对照组、实验组均呈高表达,与正常组比较差异有显著性(P<0.05);对照组、实验组与模型组比较差异有显著性(P<0.05);对照组与实验组比较差异有显著性(P<0.05).结论:透骨消痛颗粒可通过抑制COX-2和iNOS在OA早期滑膜和软骨细胞中的表达及其诱导产物PGE2和NO的生成,从而达到控制OA早期炎症、减轻疼痛、缓解症状的作用.AIM：To investgate the mechanism of influence a-bout granula of penetrating bone removeing pain intervene the ex-pression of COX-2 and iNOS in early stage of osteoarthritis by ani-mals experiment,to investgate mechanism of granula of penetrat-ing bone removeing pain on treatment of osteoarthritis.METH-ODS：We devided the New Zealand rabbits into 3 groups random-ly after replicated knee osteoarthritis with modified Hulth modeling method：model group,control group,experimental group,with the normal group were 4 groups in total.We execute all rabbits af-ter they were fed intragastically for 4 weekes.Then the following detection were taken out：General Observation of articular cavity;histological Conversation of synovium and cartilage;content of NOS,NO,test of iNOSmRNA,concentration of COX-2 and PGE2 within joint.RESULTS：After four weekes,there was sig-nificant contrast （P 〈0.05 ）of the concent of NOS,NO,iN-OSmRNA,COX-2 and PGE2 of model group,control group,ex-perimental group compared with the normal group;There was sig-nificant contrast （P 〈0.05 ）of the concent of NOS,NO,iN-OSmRNA,COX-2 and PGE2 of experimental groups and control group compared with the model group;There was significant con-trast （P 〈0.05）of the concent of NOS,NO,iNOSmRNA,COX-2 and PGE2 of experimental group compared with control group. CONCLUSION：The granula of penetrating bone removeing pain can control inflammation,relieve pains and alleviate symptoms in early stage of osteoarthritis by inhibiting the expression of COX-2 and iNOS within cartilage and synovium and releving the produc-tion of PGE2 and NO which can be inducktived by COX-2 and iN-OS.

关 键 词：透骨消痛颗粒 骨性关节炎 滑膜 软骨 COX-2 INOS NO PGE2 

分 类 号：R274.32[医药卫生—中医骨伤科学] R684.3[医药卫生—中医学]