三七皂苷R1对肝纤维化大鼠TGF-β1/Smad3信号的影响  

The Effect of Notoginsenoside R1 on TGF-β1/smad3 Signal Pathway in Hepatic Fibrosis Rats

作　　者：普显宏 何媛[2] 黄微[3] 茹金[3] 杨金伟[4] 

机构地区：[1]姚安县人民医院内一科,云南楚雄675300 [2]云南省第一人民医院消化科,云南昆明650032 [3]昆明医科大学神经科学研究所,云南昆明650032 [4]云南省第一人民医院普外二科,云南昆明650032

出　　处：《现代生物医学进展》2015年第4期622-626,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81260075;31260253);云南省科技厅-昆明医科大学应用基础研究联合专项(2012FB002;2012FB092;2013FB114);云南省肿瘤转化医学工程技术研究中心(2011DH011);云南省教育厅科学研究基金重点项目(2012Z151C)

摘　　要：目的:探讨SD大鼠肝纤维化后肝组织及血清中转化生长因子-β1(Transforming Growth Factor-β1,TGF-β1)及Smad3的表达和变化,以及三七皂苷R1对肝纤维化的保护作用。方法:72只健康雄性SD大鼠分为对照组、二甲基亚硝胺(NDMA)组和三七皂苷R1组,再按不同时间点分为1、2、4周,3个亚组,每个亚组8只动物。NDMA组采用NDMA 2 m L/kg腹腔注射,三七皂苷R1组同时静脉注射三七皂苷R1,剂量为100 mg/kg体重,对照组注射等量的生理盐水。在各组的不同时间点采用RT-PCR及ELISA技术检测肝组织及血清中TGF-β1、Smad3的表达及变化。结果:1、TGF-β1、Smad3 m RNA及蛋白在各组中均有表达。2、对照组各时间点比较均无统计学意义(P>0.05)。NDMA组中,随着损伤时间的延长,TGF-β1、Smad3 m RNA及蛋白的表达逐渐上调,且各时间点与对照组比较有统计学意义(P<0.05)。而三七皂苷R1组TGF-β1、Smad3 m RNA及蛋白在各时间点均较NDMA组表达下调,有统计学意义(P<0.05)。结论:1、TGF-β1/Smad3信号参与了肝纤维化的发生和发展过程,且随损伤的逐渐加重,表达越高。2、三七皂苷R1可降低肝组织中TGF-β1/Smad3信号的表达,减轻肝细胞的纤维化,发挥保护肝组织损伤的作用。Objective：To investigate the expression of transforming growth factor-β1 （TGF-β1） and Smad3 in liver and serum after hepatic fibrosis,and the protective effect of Notoginsenoside R1 on hepatic fibrosis.Methods：72 healthy male SD rars were randomly divided into control group,NDMA group and Notoginsenoside R1 group,according to the different time points of 1,2,4 weeks,3 subgroups,each subgroup 8 animals.For NMDA group,NMDA was administrated by intraperitoneal injection （2 mL/kg）; and for Notoginsenoside R1 group,Notoginsenoside R1 was administrated by intravenous injection with a dose of 100 mg/kg and the animals of control group was injected with equal amount normal saline.The expression of TGF-β 1 and Smad3 in liver tissues and serum was detected at different time points using ELISA and RT-PCR.Results：Expression of TGF-β1 and Smad3 protein and mRNA was detectable in all groups.In the control group,the expression of TGF-β1 and Smad3 at different time points had no statistical significance （P〉0.05）.Compared with that in the control group,the expression of TGF-β1 and Smad3 protein and mRNA in NMDA group gradually increased at each time point with the injury,and showed statistical significance （P〈0.05）.While in Notoginsenoside R1 group,expression of TGF-β1 and Smad3 at each time point was down-regulated,with statistical significance （P〈0.05） compared with that in the NDMA group.Conclusions：TGF-β1/Smad3 signal was involved in the occurrence and development of liver fibrosis,and with the aggravation of injury,the expression of TGF-β1 and Smad3 gradually increased.Notoginsenoside R1 could reduce TGF-β1/Smad3 signal in liver tissues,then reduce liver fibrosis and protect the liver from injury.

关 键 词：三七皂苷R1 肝纤维化 大鼠 转化生长因子-Β1 SMAD3 

分 类 号：Q953[生物学—动物学] R575.2[医药卫生—消化系统]