蛋白酶活化受体2促进结肠癌细胞与基质粘附  

Activation of Proteinase Activated Receptor 2 Promotes Adhesion between Colorectal Cancer Cell and ECM

作　　者：马怡茗[1] 赵新华[1] 包韩 乌云[1] 韩文晓[1] 汪红英[1] 

机构地区：[1]北京协和医学院中国医学科学院肿瘤医院肿瘤研究所分子肿瘤学国家重点实验室,北京100021

出　　处：《现代生物医学进展》2015年第5期801-803,共3页Progress in Modern Biomedicine

基　　金：国家重点基础研究发展973规划项目(2012CB967000);国家自然科学基金青年科学基金项目(81201966)

摘　　要：目的:蛋白酶活化受体2激活对结肠癌细胞HT29粘附能力的影响。方法:通过激活多肽活化细胞膜表面的蛋白酶活化受体2,检测对结肠癌细胞与FN和Matrigel粘附能力的影响。建立蛋白酶活化受2敲降的结肠癌细胞,检测对结肠癌细胞与基质粘附能力的影响。结果:蛋白酶活化受体2激活,能够促进结肠癌细胞与基质的粘附能力;而蛋白酶活化受体敲降则抑制粘附。结论:蛋白酶活化受体2参与对结肠癌细胞粘附能力的调控,其激活能够促进结肠癌细胞与基质的粘附能力。Objective： To determine the role of PAR2 in adhesion between colorectal cancer cell and extracellular matrix. Methods： Adhesion assay was performed to study the role of PAR2 in adhesion between colorectal cancer cell and fibronectin or Matrigel. PAR2 was either activated with the activating peptide or knocked down with shRNA in cancer cell lines. Results： Activation of PAR2 significantly increased the adhesion ability of cancer cell with extracellular matrix. And PAR2 knocking down impaired the adhesion between cancer cell and extracellular matrix. Conclusion： PAR2 plays an important role in cancer cell adhesion.

关 键 词：蛋白酶活化受体2 细胞粘附 结肠癌 

分 类 号：R735.35[医药卫生—肿瘤] R730.23[医药卫生—临床医学]