卵巢上皮性癌中干扰素介导的跨膜蛋白1的表达意义  

Expression and Significance of IFITM1 in Epithelial Ovarian Carcinoma

作　　者：杨蓉[1] 孙萍 宋芳霞[1] 郝炯[1] 王建[3] 

机构地区：[1]陕西省第二人民医院妇产科,陕西西安710004 [2]陕西省肿瘤医院妇瘤科,陕西西安710061 [3]第四军医大学西京医院妇产科,陕西西安710033

出　　处：《现代生物医学进展》2015年第5期839-843,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81172458)

摘　　要：目的:探讨干扰素介导的跨膜蛋白1(Interferon-induced transmembrane protein 1,IFITM1)基因在卵巢上皮性癌中表达的相关性及其意义。方法:应用Western blotting检测正常卵巢、卵巢良性肿瘤、卵巢交界性肿瘤和卵巢上皮性癌组织中IFITM1蛋白表达。免疫组织化学检测12例正常卵巢、21例卵巢良性肿瘤、18例卵巢交界性肿瘤和85例卵巢上皮性癌组织中IFITM1的蛋白表达,同时分析IFITM1表达状况与临床病理因素之间的相关性。结果:Western blotting显示卵巢上皮性癌和卵巢交界性肿瘤中IFITM1表达水平明显高于正常卵巢组织和卵巢良性肿瘤。免疫组化显示在正常卵巢组织中IFITM1阳性表达率为41.7%(5/12),在卵巢良性肿瘤组织中71.4%(15/21),在卵巢交界性肿瘤组织中为72.2%(13/18),在卵巢上皮性癌中为77.6%(66/85),IFITM1蛋白表达强度在正常卵巢、良性卵巢肿瘤、交界性卵巢肿瘤、上皮性卵巢癌间的比较有统计学意义(P<0.05)。IFITM1蛋白表达与病理类型、肿瘤分化程度、肿瘤FIGO分期有关(P<0.05),与淋巴结转移、腹水无明显相关性。化疗敏感组和耐药组的IFITM1表达强度间差异有统计学意义(P<0.05)。结论:IFITM1在正常卵巢、卵巢良性肿瘤、卵巢交界性肿瘤和卵巢上皮性癌组织中的表达依次升高,并与卵巢癌以铂类为基础的化疗耐药性产生有相关性,为进一步研究IFITM1在卵巢癌诊治及化疗中的应用前景提供依据。Objective： To investigate the role of Interferon-induced transmembrane protein 1 （IFITM1） in the carcinogenesis and evaluate its clinical significance in epithelial ovarian carcinoma. Methods： The expression level of IFITM1 was detected by the Western blot analysis in different ovaries, and the immunohistochemical method （SP） was used to determine the expression levels of IFITM1 among 12 normal ovarian tissue cases, 21 benign ovarian tumor cases, 18 borderline ovarian tumor cases and 85 ovarian carcinoma cases. Then the relationship between the expression level of IFITM1 in ovarian carcinoma and the clinicopathological features was analyzed by statistics. Results： Western blot analysis showed that the expression levels of IFITM1 differed in different ovarian tissues. The immuno- histochemical technique showed that the positive rate of IFITM1 expression was 77.6% （66/85） in ovarian carcinoma, higher than that of ovarian （41.7 %, 5/12）, benign ovarian tumor （71.4 %, 15/21） and borderline ovarian tumor （72.2 %, 13/18） with a dramatical statistic significance （P〈0.05）. The expression level oflFITM1 was correlated with pathology type, tumor grad and FIGO stage in ovrian carcinoma （P〈0.05）. The expression intensity of IFITM1 protein in ovarian carcinoma was significantly related to its chemotherapy sensitivity. Conclusion： The expression levels of IFITM1 gradually increased in normal ovarian tissue, benign ovarian tumor, borderline ovarian tumor and ovarian carcinoma. Positive expression level of IFITM1 played a vital role in carcinogenesis and progression of ovarian carcinoma including the drug-resistance to platinum-based chemotherapy. Our research can provide better evidence to the further study of IFITM1 in diagnosis and targeted treatment of ovarian carcinoma.

关 键 词：卵巢肿瘤 IFITM1基因 免疫组织化学 耐药 

分 类 号：R737.33[医药卫生—肿瘤]