XPD和ERCC1基因多态性与进展期结直肠癌患者铂剂为基础化疗方案治疗的毒副作用  

Role of Xeroderma Pigmentosum D and Excision Cross Repair Cross Complementing Gene 1 Polymorphism in the Occurrence of Side Effects for Advanced Colorectal Cancer Patients with Treatment of Platinum Based Chemotherapy

作　　者：张正华[1] 侯凯生[1] 谢芳[1] 金重华[1] 洪洁艳[1] 卫海民[1] 吴学勇[1] 

机构地区：[1]上海市静安区中心医院肿瘤科,上海200040

出　　处：《现代生物医学进展》2015年第6期1046-1049,共4页Progress in Modern Biomedicine

基　　金：上海市静安区十百千人才建设工程(201006B003)

摘　　要：目的:探讨着色性干皮病基因D(Xeroderma Pigmentosum D,XPD)和剪切修复交叉互补基因l(Excision Repair Cross Complementing Gene 1,ERCCl)多态性基因型与以铂类为基础的化疗方案治疗结直肠癌的毒副作用的关系。方法:采用聚合酶链反应--限制性片段长度多态性(Polymerase Chain Reaction-Restriction Fragment Length Polymorphism,PCR-RFLP)分析方法,对我院2010年12月至2013年12月应用含奥沙利铂方案治疗的42例汉族进展期结直肠癌患者的XPD和XRCCl的多态性基因型进行分析,比较不同基因型与临床病理因素及化疗不良反应的关系。结果:XPD、ERCC1的单核苷酸多态性(Single Nucleotide Polymorphism,SNP)分布与年龄、性别、淋巴转移、肿瘤的部位、化疗史、分化程度、器官转移个数差异无统计学意义(P>0.05);XPD基因型中,其中AA基因型以骨髓抑制、恶心呕吐为主,AG基因型以腹泻及肝肾损伤为主,GG基因型以神经毒性及口腔黏膜炎为主,差异有统计学意义(P<0.05);ERCC1基因型中,LG基因型以骨髓抑制、恶心呕吐及腹泻等症状为主,LL基因型以肝肾损伤、神经毒性及口腔黏膜炎为主,差异有统计学意义(P<0.05)。结论:XPD和ERCCl的基因型可能与结直肠癌铂类药物化疗的不良反应有关。Objective： To investigate role of Xeroderma Pigmentosum D and Excision Cross Repair Cross Complementing Gene 1 polymorphism in the occurrence of side effects for advanced colorectal cancer patients with treatment of platinum based chemotherapy.Methods： The XPD and ERCC1 gene polymorphism of 42 cases of Han patients with advanced colorectal cancer who were treated by programs with oxaliplatin in our hospital from 2010 December to 2013 December were analysed by polymerase chainreaction-restriction fragment length polymorphism（PCR-RFLP）. The relationship among different genotypes and clinical pathological factors and side effects of chemotherapy were analyzed. Results： There was no significant difference in the age, gender, lymphatic metastasis, tumor location,history of chemotherapy, differentiation, and the number of organ metastasis between single nucleotide polymorphism（SNP） distribution of XPD and ERCC1（P0.05）. Of the genotype of XPD, AA was related to the bone marrow suppression, nausea and vomiting,diarrhea;AG to liver injury; GG to neurotoxicity and oral mucositis; the differences were statistically significant（P 0.05）. Of ERCC1 genotype,LG was related to bone marrow suppression, nausea, vomiting and diarrhea and other symptoms; LL to liver and kidney damage,neurotoxicity and oral mucositis; the differences were statistically significant（P0.05）. Conclusion： Genotypes XPD and ERCCl may be associated with the occurrence of side effects for advanced colorectal cancer patients with platinum based chemotherapy.

关 键 词：结直肠癌 着色性干皮病基因D 剪切修复交叉互补基因1 化疗 不良反应 

分 类 号：R735.3[医药卫生—肿瘤]