外源添加物降低镉胁迫下香菇体内镉含量及对酶活性的影响  

Exogenous Additives Decrease in vivo Cadmium Content and Have Effect on Metabolism Enzymes of Lentinus edodes Under Cd Stress

作　　者：杨晖[1] 赵鹂[1,2] 张龙[1] 梁巧玲[1] 柯乐芹[1] 

机构地区：[1]丽水学院生态学院,浙江丽水323000 [2]浙江广播电视大学,浙江杭州310030

出　　处：《核农学报》2015年第1期183-191,共9页Journal of Nuclear Agricultural Sciences

基　　金：浙江省自然科学基金(LY12C15002)

摘　　要：香菇等可食用菇类比绿色植物体内更能逐步聚集浓度大的镉等重金属。子实体中的镉危及食用者的健康,因此减少菇类子实体中镉的含量,对人体健康意义重大。本研究检测了5种外源添加物水杨酸、沸石、石膏、壳聚糖及硫酸锌对镉胁迫下香菇体内镉含量的降低作用,并测定不同添加物对香菇羧甲基纤维素酶和漆酶活性的影响。试验结果表明,被测试的添加物中,硫酸锌对香菇808和868子实体中的镉含量著,石膏次之,表明硫酸锌是5种外源添加物中降低香菇体内镉浓度的最佳选择。此外,通过测定5种外源添加物在3 mg·kg-1镉胁迫下对香菇羧甲基纤维素酶和漆酶活性的影响,试验数据表明菌丝满袋期时,在添加硫酸锌或石膏时,香菇羧甲基纤维素酶和漆酶活性达到最高,说明培养料中添加硫酸锌或石膏在降低香菇体内镉含量的同时,也可以一定程度上增强生长代谢酶的活性。Compared with green plants,edible mushrooms including Lentinus edodes can build up large concentrations of heavy metals like cadmium. Cadmium in fruit body is potential harmful to the consumer of mushrooms. Consequently,decreasing cadmium content in fruit body is significantly important to reducing human health danger. Here,we tested decreasing effect of five kinds of exogenous additives,salicylic acid,zeolite,gypsum,chitosan and zinc sulphate,on cadmium concentration in the body of Lentinus edodes under cadium stress. The function of five kinds of exogenous additives on carboxymethyl cellulase and laccase activity was also detected. Our results showed that only two kinds of exogenous additives tested,zinc sulphate and gypsum,obviously cut down Lentinus edodes ‘808＇and ‘868＇fruit body cadmium content under 3 mg·kg- 1Cd stress. Thereinto,zinc sulphate had the most notable action on decreasing cadmium content in fruit body. These data indicate that zinc sulphate is the best choice to use as exogenous additives in reducing cadmium content in Lentinus edodes fruit body under 3 mg·kg- 1Cd stress. Moreover,the effects of five kinds of exogenous additives on enzyme activity of carboxymethyl cellulase and laccase were tested under 3 mg·kg- 1Cd stress.Data here showed that in mycelial packful stage,added with zinc sulphate or gypsum,two enzyme activities of carboxymethyl cellulase and laccase reached the peak,respectively. Results above suggest that zinc sulphate or Gypsum added into culture materials can not only reduce Lentinus edodes cadmium content in vivo but also probably increase metabolism enzyme activities. Hence,Lentinus edodes might take more advantage of cellulose and lignin in culture materials and the production of mushroom can be relatively raised.

关 键 词：香菇 体内镉含量 酶活性 外源添加物 

分 类 号：S646.12[农业科学—蔬菜学] Q945.78[农业科学—园艺学]