美沙拉秦、蒙脱石散和酪酸梭菌对溃疡性结肠炎大鼠血细胞因子的影响  

Impacts of mesalazine,montmorillonite powder,clostridium and on serum cytokine of rats with experimental ulcerative coliti

作　　者：张莹 熊晶晶[2] 黄永坤[2] 刘梅[2] 胡红卫[2] 远孟梦 李红丽[3] 魏来[2] 赵亚玲[2] 丁臻博[2] 

机构地区：[1]大理白族自治州人民医院儿科,云南671000 [2]昆明医科大学第一附属医院儿科,昆明650032 [3]昆明医科大学附属儿童医院,昆明650000

出　　处：《中国免疫学杂志》2015年第2期240-246,249,共8页Chinese Journal of Immunology

基　　金：益普生腹泻基金资助项目(IDF-2012-01)

摘　　要：目的:研究美沙拉秦、蒙脱石散、酪酸梭菌对2,4,6三硝基苯磺酸(TNBS)/乙醇法诱导的溃疡性结肠炎大鼠IL-17、IL-23、TGF-β1、IFN-γ的影响。方法:将85只大鼠随机分为模型组、美沙拉秦组、蒙脱石散组、酪酸梭菌组、美沙拉秦联合蒙脱石散组5组,每组15只;正常大鼠为空白对照组10只,一共6组。通过TNBS/乙醇法建立溃疡性结肠炎的大鼠模型。按大鼠体表面积折算药量,分别给予相应剂量生理盐水、美沙拉秦、蒙脱石散、酪酸梭菌、美沙拉秦联合蒙脱石散治疗。12 d后处死全部大鼠,收集大鼠的血标本及结肠标本。采用ELISA法测定血清中IL-17、IL-23、TGF-β1、IFN-γ的含量,分别对比6组细胞因子的水平。结果:(1)通过TNBS/乙醇法建立大鼠模型,HE染色病理切片发现大鼠结肠远端炎症明显。(2)模型组、美沙拉秦组、蒙脱石散组、酪酸梭菌组、美沙拉秦联合蒙脱石散组较空白对照组的血清IL-17、IL-23和IFN-γ含量均升高,血清TGF-β1含量均降低,差异有统计学意义(P<0.05)。(3)与模型组比较,蒙脱石散组、酪酸梭菌组、美沙拉秦组的血清IL-17、IL-23和IFN-γ含量均降低,血清TGF-β1含量均升高,差异有统计学意义(P<0.05)。(4)美沙拉秦、蒙脱石散、酪酸梭菌和美沙拉秦联合蒙脱石散的治疗效果比较,美沙拉秦和美沙拉秦联合蒙脱石散疗效较好,差异有统计学意义(P<0.05),酪酸梭菌与蒙脱石散疗效差异不明显。结论:(1)从症状体征及病理学检查表明:TNBS/乙醇法成功建立了大鼠溃疡性结肠炎模型。(2)在TNBS/乙醇法诱导的实验性溃疡性结肠炎大鼠血清中IL-17、IL-23、IFN-γ表达增高,与炎症呈正相关;结肠炎大鼠血清中TGF-β1表达降低,与炎症呈负相关。(3)美沙拉秦、蒙脱石散、酪酸梭菌、美沙拉秦联合蒙脱石散可能通过下调模型大鼠促炎因子IL-17、IL-23和调节因子IFN-γ的分泌,上调抗炎因子TGF-β1的分泌,缓解Objective：To establish the model of rats with UC by 2,4,6 trinitrobeuzene sulfonic acid （TNBS）/ethanol to understand the changes of IL-17, IL-23, TGF-β1, IFN-γ in pathogenesis of ulcerative colitis （UC） and the impacts of mesalazine, montmorillonite powder and clostridium on that. Methods ： 85 rats were divided randomly into 6 groups including the model, mesalazine, montmorillonite,clostridium, mesalazine combined with montmorillonite powder group （15 rats per group ）and an additional control group of 10 normal rats. TNBS/ethanol was used to establish the model of rats with UC. The dosage of model, mesalazine, montmorillonite powder,clostridium and mesalazine combined with montmorillonite powder were converted by surface area of rat and given to the rats by gastric-tube daily. All rats were executed at the 12th day of the molding and treatment, and the blood and colon samples were collected. The content of IL-17, IL-23, TGF-β1, IFN-γ was measured in the blood by the ELISA method. The different changes of the four cytokines were compared separately. Results： （ 1 ） TNBS/ethanol was used to establish the model of rats with UC. The obvious inflammation can be observed on distal colon of rats by pathologic sections of HE stained. （2） The levels of plasma IL- 17, IL-23 and IFN-γ in the model, mesalazine, montmorillonite, clostridium and mesalazine combined with montmorillonite powder group were higher than ones in the control gruop（ P〈0. 05 ） ,meanwhile, the levels of plasma TGF-β1 are lower （P〈0. 05 ）. 3. Comparing with the model group, the levels of plasma IL-17, IL-23 and IFN-γ in the mesalazine, montmorillonite, clostridium and mesalazine combined with montmorillonite powder group were lower, and the levels of TGF-β1 were higer. The differences have statistic significative （ P〈 0.05 ）. （4） Comparing the efficacy of treatment among mesalazine, montmorillonite powder, clostridium and mesalazine combined with montmorillonite powder, mesalazine and mesalazine comb

关 键 词：美沙拉秦 蒙脱石散 酪酸梭菌 溃疡性结肠炎 细胞因子 

分 类 号：R574.62[医药卫生—消化系统]