海水pH对颗石藻生长以及二甲基硫产生的影响  

The Influence of Seawater pH on the Growth and Dimethylsulfide Production of Emiliania huxleyi

作　　者：于娟[1] 赵丽军[1] 杨桂朋[1] 田继远[2] 刘伟[3] 许超平[1] 

机构地区：[1]中国海洋大学海洋化学理论与工程技术教育部重点实验室,山东青岛266100 [2]青岛农业大学食品科学与工程学院,山东青岛266109 [3]中国科学院海洋研究所,山东青岛266071

出　　处：《中国海洋大学学报（自然科学版）》2015年第2期72-79,共8页Periodical of Ocean University of China

基　　金：国家自然科学基金创新研究群体项目(41221004);国家自然科学基金重大国际合作研究项目(41320104008);国家自然科学基金项目(41030858;41106122);山东省自然科学基金项目(ZR2011DQ005);国家海洋局近岸海域生态环境重点实验室资助项目(201306)资助

摘　　要：海洋酸化是目前海洋环境所面临的严峻问题之一,而钙化藻-颗石藻(Emiliania huxleyi)是大洋中二甲基硫(Dimethylsulfide,DMS)产生的主要藻种。本文初步研究了3种海水pH(8.1、7.9、7.7)对颗石藻生长、细胞直径以及DMS/DMSP(Dimethylsulfoniopropionate,二甲基巯基丙酸内盐)产生的影响。研究结果表明3种pH(8.1、7.9、7.7)条件下颗石藻的细胞密度、比生长率没有显著差异,颗石藻培养第10天的扫描电镜细胞形态以及藻细胞直径测定结果显示,pH=7.9和pH=7.7的颗石藻直径比pH=8.1的颗石藻直径显著降低;颗石藻DMS总量、单细胞DMS/单细胞DMSP产量在3种pH(8.1、7.9、7.7)中两两之间没有显著差异;而pH=7.7的DMSP总量显著低于pH=8.1的DMSP总量。Pearson相关分析结果表明,细胞分裂导致3种pH的单细胞DMSP含量与细胞密度、比生长率均呈负相关,3种pH的总DMS/总DMSP含量与细胞密度均呈正相关。CO2浓度升高引起的海洋酸化不仅导致pH降低,而且海水中的碳酸盐体系也会发生变化,因此本实验结果外推到现实环境时还要考虑碳酸盐体系变化对DMS产生的影响。Ocean acidification is one of the serious problems currently faced by marine environment, and calcifid alga- Emiliania huxleyi is the primary alga to produce dimethylsulfide （DMS） in the ocean. This paper preliminary investigated the changes of the growth, cell diameter and the production of DMS and dimethylsulfoniopropionate （DMSP） for E. huxleyi at three levels of pH （8. 1, 7.9 and 7.7）. The results showed that the cell densities and specific growth rates among three pH levels had no significant effects, respectively. Cell morphology observation results in scanning electron microscopy （SEM） at day 10 and the diameter results of E. huxleyi indicated that the diameters of pH= 7.7 and pH= 7. 9 were obviously lower than those of pH = 8.1. Total DMS, cellular DMS/cellular DMSP production of E. huxleyi among three pH levels （8. 1, 7.9, 7.7） had no significant effects; while total DMSP concentrations of pH=7. 7 were significantly lower than those of pH=8. 1. Pearson correlation between cellular DMSP contents and specific growth rates/cell densities was negative, which might be due to cell lysis. There were positive correlations between total DMS/total DMSP contents and cell densities for three levels of pH. Increasing CO2 not only caused the decrease of pH, but also changed the carbonate system in the seawater. Therefore, when we put these results to the natural environment, the effects of carbonate system and food chain changes on the DMS production should be considered.

关 键 词：颗石藻 PH DMS DMSP 

分 类 号：P734.5[天文地球—海洋化学]