“从肠论治”对哮喘小鼠BALF炎症细胞及血清IgE含量的影响  

Effect of Relaxing the Large Intestine on BALF inflammatory cell and Serum IgE in Asthma Mices

作　　者：刘妙[1] 郑丰杰[1] 高誉珊[1] 张淑静[1] 张天宇[1] 孙燕[1] 李宇航[1] 

机构地区：[1]北京中医药大学基础医学院,北京100029

出　　处：《世界中医药》2015年第1期26-29,共4页World Chinese Medicine

基　　金：国家重点基础研究发展计划(973计划)资助项目(编号:2009CB522704);北京中医药大学"经方现代应用关键科学问题的基础研究"创新团队(编号:2011-CXTD-04)

摘　　要：目的:观察"从肠论治"对哮喘小鼠支气管肺泡灌洗液中嗜酸性粒细胞、中性粒细胞及血清Ig E含量的影响,为探讨"肺与大肠相表里"理论提供实验依据。方法:采用卵清蛋白(OVA)致敏激发方法制备过敏性哮喘小鼠模型,芒硝灌胃刺激,进行小鼠气道反应性试验,肺组织形态学观察,肺支气管肺泡灌洗液细胞分类计数、血清Ig E含量检测。结果:与正常组相比,OVA组小鼠气道阻力增加,肺组织出现炎性病理改变,肺泡灌洗液中嗜酸性粒细胞、淋巴细胞总数明显增加,血清Ig E含量均明显升高;经芒硝从肠干预后,芒硝组小鼠气道阻力明显降低,肺组织形态结构有一定程度的改善,肺泡灌洗液中嗜酸性粒细胞、淋巴细胞总数明显减少,血清Ig E含量显著降低。结论:芒硝刺激大肠,有助于改善气道通气功能,抑制哮喘小鼠气道炎症,改善气道免疫微环境。Objective：To explore the biological foundation for the TCM theory of the interior-exterior relationship between the lung and the large intestine, we conducted an experiment to evaluate the content of eosinophils and neutrophils in bronchoalveolar lavage fluid （ BALF） and IgE in blood serum after the asthma modeled mice were intervened with mirabilite at large intestines.Methods：Asthma model mice were established by OVA sensitization.Airway responsiveness, histopathology change, classification and counting of the cells of BALF and content of blood serum IgE were observed after large intestine was stimulated by mirabilite.Re-sults：Compared with the normal rats, the asthma model rats’airway resistance increased obviously, the number of eosinophils and neutrophils in BALF and IgE in blood serum significantly increased.After intervened with mirabilite, airway resistance decreased obviously, eosinophils and neutrophils in BALF and IgE in blood serum significantly lowered.Conclusion： Stimulating the large intestine by mirabilite showed beneficial effects in alleviating airway responsiveness and airway inflammation, and improving airway immune microenvironment in asthma mice model.

关 键 词：从肠论治 肺合大肠 支气管哮喘 

分 类 号：R223.1[医药卫生—中医基础理论]