半枝莲提取物对黄曲霉素B_1所诱发肝癌Notch1的影响  

Effects of Banzhilian Extract on Notch1 of Aflatoxin B_1-induced Hepatic Carcinoma

作　　者：蔡林雪[1] 刘澍楠 

机构地区：[1]福建医科大学药学院药物分析系,福州350004 [2]福州总院四七六医院,福州350001

出　　处：《世界中医药》2015年第1期83-85,共3页World Chinese Medicine

基　　金：福建省自然科学基金项目(编号:2012J01131)

摘　　要：目的:探讨半枝莲提取物(ESB)对黄曲霉素B1所诱发的肝癌大鼠Notch1的影响。方法:选取60只Wistar大鼠,随机分为空白组、模型组、半枝莲提取物低、高剂量组,每组15只。建立用黄曲霉素B1所诱发的肝癌大鼠的动物模型。其中空白组与模型组给予蒸馏水灌胃,半枝莲提取物低、高剂量组给予不同浓度的ESB灌胃治疗。灌胃1次/d,连续治疗1个月。治疗结束后将所有大鼠处死,观察各组大鼠的体质量、肝表面的结节数,并取肝组织采用免疫组化法检测肝组织中Notch1的表达情况。结果:与空白组比较,模型组和ESB低、高剂量组大鼠的体质量明显降低(P<0.05),肝表面的结节数明显增多(P<0.05),Notch1阳性细胞数明显升高(P<0.05);与模型组比较,ESB低、高剂量组大鼠的体重升高(P<0.05),肝表面的结节数及Notch1阳性细胞数明显降低(P<0.05)。结论:低、高剂量的半枝莲提取物能够有效的抑制肝癌的恶化,其机制可能与下调肝组织中Notch1有关。Objective：To investigate the Banzhilian extract’s effects on Notch1 of rats with aflatoxin B1-induced hepatic carcino-ma.Methods：Sixty Wistar rats were randomly divided into 4 groups：normal control group,hepatocarcinoma model group,extract’ s high dose group and low dose group.Hepatic carcinoma model were induced by aflatoxin B1.The control group and the model group were given distilled water lavagely, the extract ’s groups were given different concentrations of extracts lavagely, qd.for a month.All rats were killed in the 4th week,measure weight,counted number of nodules on the surface of liver.Immunohistochemis-try were used to detect the expression of Notch1.Results：Compared with the control group,the rats’weight in other groups dropped significantly （ P〈0.05） ,while the nodule number and Notch1 levels increased significantly （ P〈0.05）;Compared with the mod-el group,the rats’weight in the extract’s of high dose group and low dose group were increased （ P〈0.05） ,while the nodule num-ber and Notch1 levels were reduced considerably（P〈0.05）.Conclusion：Banzhilian extract could inhibit aggravation of liver car-cinoma,which is probably related to the down-regulating of liver Notch1.

关 键 词：半枝莲提取物 黄曲霉素B1 肝癌 NOTCH1 

分 类 号：R284.2[医药卫生—中药学] R273[医药卫生—中医学]