磷酸化ERK-1及磷酸化AKT在子宫内膜癌中表达及意义  

Expression and significance of ERK-1 and AKT in human endometrial carcinoma

作　　者：骆向丽 付乐凡 付斌[2] 时志民[2] 肖艳莉[2] 孙冬霞[3] 席丰[2] 沈艳峰[2] 王蕾[2] 

机构地区：[1]河北省曲周县妇幼保健院,河北曲周057250 [2]河北工程大学,河北邯郸055901 [3]河北省邯郸市妇幼保健院,河北邯郸055902

出　　处：《现代中西医结合杂志》2015年第8期820-822,共3页Modern Journal of Integrated Traditional Chinese and Western Medicine

摘　　要：目的观察子宫内膜癌组织中磷酸化ERK-1（P-ERK-1）及磷酸化AKT（PAKT）表达情况。方法采用免疫组织化学S-P法检测78例子宫内膜癌组织、40例正常子宫内膜组织中P-ERK-1和P-AKT的表达情况。结果子宫内膜癌组织中P-ERK-1和P-AKT表达定位于细胞核和细胞膜,其表达率分别为74%（58/78）和77%（60/78）,明显高于正常子宫内膜组织（P均〈0.05）;子宫内膜癌组织中,P-ERK-1和P-AKT蛋白在组织学分级G2、G3级及病理分期Ⅱ、Ⅲ-Ⅳ期的表达率分别高于G1级和Ⅰ期（P均〈0.05）,与肌层浸润程度有关（P〈0.05）;P-ERK-1表达与无淋巴结转移、病理组织类型无关（P均〉0.05）;P-AKT与有无淋巴结转移有关（P〈0.05）,而与病理组织类型无关（P〉0.05）;相关性分析显示,二者之间呈正相关（P〈0.05）。结论 P-ERK-1和P-AKT在子宫内膜癌组织中过度表达,与子宫内膜癌的发生、发展和转移密切相关。Objective It is to investigate the expressions of ERK- 1 （P- ERK- 1 ） and AKT（ P- AKT） in human endo- metrial carcinoma. Methods Using SP immunohistochemistry method, the expression of P - ERK - 1 and P - AKT in the specimens of 78 cases with endometrial carcinoma and 40 cases with normal endometrium were determined. Results The ex- pressions of P - ERK - 1 and P - AKT in human endometrial carcinoma were located with the nucleus and the cell membrane. The positive expression rates were 74% （58/78） and 77% （60/78） respecitively, which were significantly higher than that in normal tissue （P 〈 0.05 ）. In the tissue of endometrial carcinoma, the expression of P - ERK - 1 and P - AKT inmoderate- lypoorly differentiated（ G2 - G3） and stage Ⅱ -Ⅲ cases was significantly higher than that in well differentiated （ G1 ） and stage I cases（ P 〈 0.05 ）, and were related with myometrial invasion（ P 〈 0.05 ）, and they were not related with pathology pattern of organization（P 〉 0.05） ; they were positively correlated with each other （P 〈 0.05 ）. Conclusion P - ERK - 1 and P - AKT were highly expressed in the endometrial cancinoma and their expressions were related with the development and the me- tastasis of this cancer.

关 键 词：子宫内膜肿瘤 磷酸化ERK-1 P-AKT 免疫组化 

分 类 号：R737.33[医药卫生—肿瘤]