P53凋亡刺激蛋白1在完全性葡萄胎中的表达及意义  

Expression and significance of ASPP1 in complete hydatidiform mole

作　　者：郝志敏[1] 朱虹[1] 

机构地区：[1]宁波大学医学院附属医院妇产科,浙江宁波315000

出　　处：《全科医学临床与教育》2015年第1期11-14,F0003,共5页Clinical Education of General Practice

摘　　要：目的探讨P53凋亡刺激蛋白1(ASPP1)在正常绒毛组织及葡萄胎中的表达,分析其与葡萄胎恶变的相关性。方法选择50例完全性葡萄胎组织作为研究组,正常早孕绒毛组50例作为对照组,采用免疫组化Envision二步法检测ASPP1蛋白的表达,回顾性分析病例组临床病理资料,判断ASPP1与葡萄胎恶变及高危因素的相关性。结果ASPP1主要表达于细胞滋养细胞及中间型滋养细胞的细胞质及细胞膜。完全性葡萄胎组中ASPP1强表达率明显低于正常早孕绒毛组(χ2=11.63,P<0.05),恶变葡萄胎ASPP1强表达率与非恶变葡萄胎比较,差异具有统计学意义(χ2=5.25,P<0.05)。ASPP1蛋白表达与子宫体积大小、血HCG值均呈负相关(r分别=-0.36、-0.31,P均<0.05),而ASPP1蛋白表达与葡萄胎患者的年龄及有无合并卵巢黄素化囊肿无明显相关性(r分别=-0.10、-0.05,P均>0.05)。轻度增生葡萄胎ASPP1强表达率均分别明显高于中度增生和重度增生(χ2分别=4.82、6.44,P均<0.05),中度增生与重度增生之间ASPP1强表达率比较,差异无统计学意义(χ2=0.04,P>0.05)。结论 ASPP1可能通过诱导凋亡在葡萄胎的发病机制及葡萄胎恶变中发挥作用,有可能作为判断预后的参考指标之一。Objective To explore apoptosis stimulating protein 1（ASPP1） expression in complete hydatidiform mole（CHM） and normal early pregnant villous and assess the association between ASPP1 expression and CHM malignant transformation. Methods The expressions of ASPP1 protein in 50 complete hydatidiform moles and 50 normal pregnant villous were detected by the method of Envision immunohistochemistry. Their clinicalpathological data were retrospectively analyzed to identify the relevance between ASPP1 expression and CHM malignancy and its high risk factors. Results ASPP1 immunoreactivity was predominantly found in the cytoplasm and cell membrane of the villous cytotrophoblasts and intermediate trophoblasts. The expression of ASPP1 protein was significantly lower in CHM than that in normal early pregnant villous（χ^2=11.63,P 0.05）. The expression of ASPP1 protein in CHM with malignant transformation was significantly lower than that in CHM with non-malignant transformation（χ^2=5.25,P〈0.05）. The expression of ASPP1 protein was negatively correlated to uterus size and HCG value（r=-0.36,-0.30,P〈0.05） while not related with age and theca lutein ovarian cyst（r=-0.10,-0.05,P〈0.05）. The expression of ASPP1 protein in CHM with mild hyperplasia of trophoblast was significantly higher than that in CHM with moderate and severe hyperplasia of trophoblast（χ^2=4.82, 6.44, P〈0.05）, but there was no statistical difference between mediate and severe hyperplasia of trophoblast（χ^2=0.04,P〉0.05）. Conclusion Down-regulation of ASPP1 may be involved in the pathogenesis and progress of CHM, probably through its effect on apoptosis. It can be as the reference index of prognosis.

关 键 词：凋亡 凋亡刺激蛋白1 妊娠滋养细胞疾病 

分 类 号：R737.33[医药卫生—肿瘤]