输合配穴针刺对LPS脑损伤幼鼠模型脑组织S100B、NSE表达的影响  

Effects of combined transport acupuncture on the expression of S100B,NSE and LPS of brain tissue of young rats with cerebral injury model

作　　者：胡晓丽[1] 王雪峰[1] 庞伟[2] 韩洋[3] 马贤德[4] 

机构地区：[1]辽宁中医药大学附属医院儿科,沈阳110032 [2]佳木斯大学儿童神经康复实验室,黑龙江佳木斯154007 [3]辽宁中医药大学研究生学院,沈阳110032 [4]辽宁中医药大学实验中心

出　　处：《中国中西医结合儿科学》2015年第1期20-22,共3页Chinese Pediatrics of Integrated Traditional and Western Medicine

基　　金：辽宁省教育厅基金资助(L2012336)

摘　　要：目的探讨输合配穴针刺干预对LPS脑损伤幼鼠模型脑组织S100B、NSE表达的影响。方法将60只SD新生的乳鼠随机分为针刺干预组、模型组和对照组,对干预组采用输合配穴针刺21d后,对各组大鼠脑组织中S100B、NSE含量进行检测。结果干预组S100B、NSE的表达明显高于对照组(P<0.01),且21d时均低于模型组,差异有统计学意义(P<0.05)。结论通过对脑损伤模型脑组织S100B、NSE表达检测,明确"输合配穴"针刺法在早期干预有效性。Objective To research the effect of acupuncture intervention on the expression of SIOOB. NSE of different types of brain injury model in rat brain tissue. Methods According to the model preparation method is divided into premature intervention group and non intervention group, LPS premature intervention group and non treatment group, Isehemia and hy- poxia group and untreated group, LPS intervention group and the non intervention group, the normal group. The method of enzyme-linked immunosorhent assay （ELISA） was used to detect S100B,NSE content in brain tissue of rats in different group. Results Rats S100B, NSE expression was closely correlated with the severity of brain injury. The expression of S100B,NSE in model group, was significantly higher than that in the blank control group （P〈0.01）. The results showed., the expression of S100B,NSE in brain tissue was lower than non intervention group expression in the acupuncture intervention group on twenty- first day. Conclusions The premature delivery to hypoxia and ischemia, rats, lipopolysaccharicle infection detection of brain in- jury. S100B,NSE are not consistent in various model,maybe that related to the severity of brain injury related model. Brain tis- sue S100B,NSE expression was lower than non intervention group expression in the acupuncture group, which can demonstrate the use of ＂shu and he point＂ early intervention of acupuncture effectively.

关 键 词：脑损伤 造模 S100B NSE 动物 实验 幼鼠 

分 类 号：R-33[医药卫生]