中药槌果藤下调p-mTOR抑制肾癌细胞侵袭和迁移  

Capparis spinosa L. Inhibit the Invasion and Metastasis of Renal Cell Carcinoma through Down-regulation of p-mTOR

作　　者：林海[1] 严杰[2] 应旭旻 

机构地区：[1]浙江中医药大学,浙江杭州310053 [2]解放军第一一七医院,浙江杭州310004 [3]杭州市急救中心,浙江杭州310021

出　　处：《云南中医学院学报》2015年第1期27-30,共4页Journal of Yunnan University of Traditional Chinese Medicine

摘　　要：目的探讨中药槌果藤对肾癌细胞生物学行为的影响及其相关作用机制。方法将不同浓度的槌果藤与肾癌细胞共培养,利用MTT、Transwell小室、western blot法检测槌果藤对肾癌细胞增殖、侵袭、迁移能力以及mTOR、p-mTOR蛋白表达的影响。结果槌果藤可显著抑制肾癌细胞的增殖、侵袭和迁移,并呈时间和浓度依赖性,10、20、40μg/mL浓度可显著下调p-mTOR表达水平(P<0.05,P<0.01,P<0.01),但对mTOR表达无影响(P均大于0.05)。结论槌果藤通过下调p-mTOR表达抑制肾癌细胞增殖、侵袭和迁移。Objective To investigate the effect of Capparis spionosaL, on the proliferation, invasion and metastasis on the renal cell carcinoma 786-0 cells and reveal the mechanisms. Methods The cells were treated with different concentrations of Capparis spionosaL. The proliferation, invasion and metastasis were detected by MTF, Transwell assay and the level of mTOR and p- mTOR protein was detected by Western blot. Results Capparis spionosaL, inhibited the proliferation, invasion and metastasis of 786- 0 cells depend on the time and drug concentration, 10, 20, 40ug/ml of the drug can significantly down-regulated the level of p- mTOR protein（P〈0.05, P〈0. 01, P〈0.01 ）, but the drug has no effect on the expression of the mTOR protein（P〉0.05）. Conclusion Capparis spionosaL, inhibit the proliferation, invasion and metastasis of 786-0 cells may through down-regulated the level of p- mTOR protein.

关 键 词：槌果藤 肾癌细胞 MTOR 侵袭 迁移 

分 类 号：R285.5[医药卫生—中药学]