FGF-23(R176Q)过表达对成年小鼠下颌骨形成和矿化的影响  

The effective of FGF-23(R176Q)overexpress in adult mouse mandible formation and mineralization

作　　者：刘洪[1] 孙雯[2] 郑阳玉[3] 

机构地区：[1]盐城卫生职业技术学院口腔医学技术教研室,江苏盐城224005 [2]南京医科大学解剖学系,210029 [3]南京医科大学口腔医学研究所,210029

出　　处：《重庆医学》2015年第7期888-890,共3页Chongqing medicine

基　　金：2012年盐城市医学科技发展计划基金资助项目(YK2012029)

摘　　要：目的研究FGF-23(R176Q)过表达对成年小鼠下颌骨形成和矿化的影响。方法使用CT扫描、血清学检查、HE染色、免疫组织化学法染色和RT-PCR等方法分析FGF-23(R176Q)转基因小鼠(TG组)和野生型小鼠(WT组)下颌骨,比较血清钙、磷浓度变化和下颌骨骨基质和矿化的差异。结果 WT组小鼠血清钙、磷和1,25(OH)2D3浓度均明显高于TG组,差异有统计学意义(P<0.05);TG组的成骨细胞密度低于WT组,差异有统计学意义(P<0.05);TG组的二聚糖阳性百分比高于WT组,而DSP阳性百分比低于WT组,差异有统计学意义(P<0.05);WT组的OCN和Ⅰ型胶原mRNA表达水平均明显高于TG组,差异有统计学意义(P<0.05)。结论 FGF-23(R176Q)过表达能够抑制下颌骨骨基质的形成,减少骨质的矿化,进而导致了下颌骨发育障碍。Objective To investigate the effective of FGF-23（R176Q） overexpression of adult mouse mandible formation and mineralization. Methods CT scanning, serological examination, HE staining,immunohistochemical staining and RT-PCR analysis were used to compared mandible of FGF-23（R176Q） transgenic mice（TG group） and wild type mice（WT group）, serum calcium and phosphorus concentrations mandibule formation and mineralization were analysised. Results Serum calcium,phosphorus and 1, 25 （OH）2 D3 concentrations in WT group were significantly higher than that in TG group,the difference was statistically significant （P〈0.05） ;the Ob. s/B. Pm TG group was lower than that of WT group, the difference was statistically significant（P〈0.05） ;the positive percentage of Biglycan in TG group was higher than that in WT group, the difference was statistically significant （P〈 0.05） ;the OCN and type I collagen mRNA levels in the WT group were significantly higher than that in TG group, the difference were statistically significant（P〈0.05）. Conclusion FGF-23（R176Q） overexpression can inhibit the formation of mandible,reduce the formation of mineralized,and reduce the development of the mandible.

关 键 词：FGF-23 过表达 矿化 骨基质 

分 类 号：R780.2[医药卫生—口腔医学]