纳米炭吸附多西紫杉醇在乳腺癌区域淋巴化疗中的临床研究  

Nano carbon adsorption taxotere clinical research in regional lymphatic chemotherapy of breast cancer

作　　者：李伟[1] 曾晓华[1] 徐发良[1] 熊忠讯[1] 罗杰[1] 

机构地区：[1]重庆市肿瘤研究所乳腺外科,400030

出　　处：《重庆医学》2015年第7期907-909,912,共4页Chongqing medicine

基　　金：重庆市卫生局基金资助项目(2012-2-490)

摘　　要：目的 探讨纳米炭吸附多西紫杉醇（TAX-CNP）淋巴化疗药物的靶向性、合理注射时间及短期疗效。方法 淋巴靶向化疗试验组（A组）：注射1mL多西紫杉醇（TAX）加1mL纳米炭（CNP）,平均分4个小组（A1~4组）,术前TAX-CNP分别在12、24、36及48h行淋巴靶向化疗后再行手术;外周静脉化疗对照组（B组）：静脉输注含1mL TAX的0.9%氯化钠溶液100mL,平均分4个小组（B1~4组）,根据上述相同的时间点经外周静脉输注相同剂量的TAX。术毕取腋窝淋巴结,用高效液相色谱仪检测两组患者淋巴结TAX的含量;将两组患者有癌转移的淋巴结行原位末端标记法（TUNEL）及流式细胞仪检测细胞凋亡情况。结果 （1）A组淋巴结TAX含量明显高于B组（P〈0.05）;（2）A1组细胞凋亡指数（AI）及凋亡率均明显低于A2、A3、A4组（P〈0.05）,A2、A3、A4组细胞AI及凋亡率比较差异均无统计学意义（P〉0.05）;（3）A组细胞AI及凋亡率均明显高于B组（P〈0.05）。结论 TAX-CNP可以显著提高淋巴结的药物浓度,具有较好的淋巴靶向性;术前24h注射系较为理想的注射时间;TAX-CNP可以明显提高转移淋巴结细胞凋亡的发生,降低短期复发率。Objective To explore the nano carbon adsorption taxotere（TAX-CNP） of the lymph node chemotherapy drug targeting property. Methods The lymph node targeted chemotherapy experimental group（A group） ： Injection of 1 mL taxotere （TAX） plus 1 mL carbon nanoparticles（CNP）, average four groups, preoperative on 12,24,36 and 48 h, the lymph node targeted chemotherapy of TAX-CNP before surgery; peripheral venous chemotherapy in the control group （13 group） ： intravenous infusion with 1 mL TAX of 0.9 % sodium chloride solution 100 mL,average four groups, according to the same point in time the peripheral intravenous doses of the same TAX. Gather the axillary lymph nodes after the surgery, used high performance liquid chromatograph tests the content of TAX in the two groups of lymph nodes;TUNEL method and flow cytometry instrument detect cell apoptosis, which are carcinoma metastasis lymph nodes of Two groups. Results （1）lymph node TAX level of group A was significant higher than group B（P〈0.05） ; （2） Cell apoptosis index（AI） and apoptosis rate of A1 group were lower than that in group A2, A3, A4 （P d0.05）, in the cell AI and apoptosis rate of A2, A3, A4 group had no statistically significant difference（P〈0.05 ） ; （3） Cells AI and apoptosis rate of cancer metastasis lymph node of Group A were significant higher than group B（P〈0.05）. Conclusion TAX-CNP can significantly increase the drug concentration of lymph nodes,which have better lymphatic targeting property;Preoperative 24 h injection is a more ideal injection time; TAX-CNP can obviously increase the metastasis lymph cell apoptosis, which can reduce short-term recurrence rate.

关 键 词：乳腺肿瘤 淋巴化疗 纳米炭 多西紫杉醇 

分 类 号：R737.9[医药卫生—肿瘤]