牙髓干细胞MHC分子表达与体外混合淋巴细胞的增殖  

MHC molecule expression in dental pulp stem cells and mixed lymphocyte proliferation in vitro

作　　者：聂姗姗[1] 刘佳[1] 张瑞涵[1] 王璇[1] 李伯琦[1] 孙大磊[1] 热甫卡提[1] 刘奕杉[1] 

机构地区：[1]新疆医科大学第一附属医院儿牙预防保健科,新疆维吾尔自治区乌鲁木齐市830054

出　　处：《中国组织工程研究》2014年第50期8162-8167,共6页Chinese Journal of Tissue Engineering Research

基　　金：新疆维吾尔自治区重点实验室医学动物模型研究实验室开放课题(XJDX1103-2013-04)~~

摘　　要：背景:若牙髓干细胞诱导分化后仍然具有与未分化时相似的免疫调节能力,则有可能为组织工程提供同种异体种子细胞来源。目的:观察牙髓干细胞表面免疫分子的表达以及体外调节淋巴细胞反应的功能。方法:从C57BL/6小鼠牙髓组织中分离获取牙髓干细胞,体外培养至第2代,流式细胞仪检测免疫分子MHC-Ⅰ、MHC-Ⅱ的表达。以1×105/孔牙髓干细胞刺激异体淋巴细胞,观察细胞增殖情况。以1×105/孔数量的牙髓干细胞或经γ-干扰素作用后的牙髓干细胞加入混合双向淋巴细胞反应体系中,观察淋巴细胞增殖情况。结果与结论:牙髓干细胞表达MHC-Ⅰ类分子,但未检测到MHC-Ⅱ类分子阳性表达。γ-干扰素刺激48 h后,MHC-Ⅰ表达未见明显增高,MHC-Ⅱ类分子表达明显增高。异体或经γ-干扰素作用的牙髓干细胞均未能刺激淋巴细胞体外增殖。说明牙髓干细胞可在体外调节淋巴细胞增殖反应,有可能成为组织工程或细胞治疗中同种异体细胞来源。BACKGROUND： If differentiated dental pulp stem cells have immune adjustment ability similar to undifferentiated ones, they could become a new source of allogeneic seed cells in tissue engineering. OBJECTIVE： To study the immunological properties of dental pulp stem cells and their immunomodulatory effects on lymphocytes in vitro. METHODS： Dental pulp stem cells were isolated from mouse dental pulp tissue. Cells at passage 2 were detected using flow cytometry for the expression of MHC-Ⅰ, MHC-Ⅱ. Inguinal lymphcells were cultured with allogeneic dental puip stem cells at a density of 1 ×105 cells per well. Dental pulp stem cells at 1×105cells per well or interferen-y-treated dental pulp stem cells were added into the mixed lymphocyte reaction system to observe lymphocyte proliferation. RESULTS AND CONCLUSION： Flow cytometry showed that undifferentiated dental pulp stem cells expressed MHC-Ⅰ but not MHC-Ⅱ. After intervention with interferen-y for 48 hours, increased expression of MHC-Ⅰ could not been seen in the dental pulp stem cells, but MHC-Ⅱ expression was increased obviously. No lymphocyte response was induced by allogeneic or interferon-y-treated dental pulp stem cells as stimulators. Experimental findings suggest that dental pulp stem cells can modulate the proliferation of lymphocytes, which may be expected to become a new source of allogeneic seed cells in tissue engineering or cell therapy.

关 键 词：干细胞 牙髓 淋巴细胞培养试验 混合 培养 牙髓干细胞 混合淋巴细胞培养 MHC抗原 小鼠 

分 类 号：R394.2[医药卫生—医学遗传学]