海南萝芙木提取物对葡聚糖硫酸钠小鼠结肠炎作用研究  

The role of extractive from Rauvolfiaverticillata(Lour. ) Baill. var. hainanensis Tsiang in mice with dextran sulphatesodium-induced ulcerative colitis

作　　者：苗新普[1] 孙晓宁[1] 崔路佳[1] 邓桃枝[1] 曹芹芳[1] 

机构地区：[1]海南省人民医院消化内科,海口570311

出　　处：《中华临床医师杂志（电子版）》2015年第4期84-88,共5页Chinese Journal of Clinicians(Electronic Edition)

基　　金：国家自然科学基金项目(81360603);海南省自然科学基金项目(813215)

摘　　要：目的探讨海南萝芙木提取物对葡聚糖硫酸钠(DSS)小鼠结肠炎的作用及机制。方法 80只BALB/c小鼠随机分为:(1)正常对照组;(2)模型组;(3)模型+柳氮磺胺嘧啶治疗组;(4)模型+海南萝芙木提取物治疗组。实验结束后剖取各组小鼠结肠炎症组织,HE染色,组织学评分;免疫组化方法检测各组小鼠肠黏膜组织中NF-κB抑制蛋白(IκB-α)阳性细胞表达率,ELISA方法检测定小鼠结肠组织白细胞介素-4(IL-4)、白细胞介素-13(IL-13)表达。结果柳氮磺胺嘧啶治疗组和海南萝芙木提取物治疗组小鼠一般情况好于DSS模型组,DAI积分低于DSS模型组;结肠组织显微镜检查,动物结肠炎症程度低于DSS模型组;动物结肠黏膜细胞中IκB-α阳性细胞表达率显著高于DSS模型组;小鼠结肠组织中IL-4、IL-13水平均显著高于DSS模型组。结论海南萝芙木提取物果胶多糖可能通过于预IκB-α蛋白表达,进而抑制溃疡性结肠炎症细胞中NF-κB活性,抑制IL-4、IL-13等炎症因子,产生抗炎效果。Objective To investigate the effects and explore the regulating mechanism of extractive from Rauvolfiaverticillata （Lour.） Baill. var. hainanensis Tsiang in the mechanism of DSS induced Mice Colitis. Methods 80 BALB/c mice were randomly divided into 4 groups：（1）normal control group;（2）model group;（3）model plus salicylazosulfa-pyridine （SASP, used as a positive control drug） （n=20/group）;（4）model plus extractive from Rauvolfiaverticillata （Lour.） Baill. var. hainanensis Tsiang. After the end of the experiment, each murine colitis tissue section was taken, HE staining;histopathological damage and histological scores were observed; With immunohistochemical staining IκB-α expression in colon tissues were detected; With enzyme-linked immunosorbent assay （ELISA） interleukin-4, interleukin-13 levels in colon tissue were measured. Results Compared with the model plus pecticpolysaccharides group and model plus SASP group, the DAI value was significantly greater in mice treated with DSS （P〈0.05）. Compared with model group, the colitis symptoms were relieved and IκB-αprotein was increased in mice of the model plus pecticpolysaccharides group and model plus SASP group （P〈0.05）. The production of IL-4 and IL-13 were increased in mice of the model plus pecticpolysaccharides group and model plus SASP group （P〈0.05）. Conclusion Pecticpolysaccharides extracted from Rauvolfia verticillata （Lour. ）Baill. var. hainanensis Tsiang ameliorated ulcerative colitis and might exhibit its anti-inflammatory effects via increased expression of IκB-α proteins and suppressing NF-κB translocation and interleukin-4 and interleukin-13 levels.

关 键 词：萝芙木 结肠炎 溃疡性 白细胞介素4 白细胞介素13 

分 类 号：R574.62[医药卫生—消化系统]