人大肠癌癌旁不同部位组织细胞外基质分子和细胞极性分子表达差异比较  

Expression of extracellular matrix and cell polarity proteins in tissues at different distances from colorectal cancer lesions

作　　者：刘红[1] 文彬[1] 陈丹[2] 刘金元[3] 胡丰良[2] 

机构地区：[1]广州中医药大学脾胃研究所,广东省广州市510405 [2]广州中医药大学第一附属医院肛肠科,广东省广州市510405 [3]广州中医药大学,广东省广州市510405

出　　处：《世界华人消化杂志》2015年第1期163-169,共7页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.81173257;中央财政支持地方高校发展专项资金"重大难治性脾胃病防治协同创新平台"基金资助项目;No.财教[2013]338号~~

摘　　要：目的:通过对肿瘤微环境中相关成分的检测,探索肿瘤微环境对大肠癌启动的意义,方法:在距离大肠癌病灶近端10、5、2 cm处分别取得组织样本,依次命名为1、2、3号位组织样本.通过常规石蜡切片-HE染色方法和免疫组织化学检测3个部位中I型胶原(collagen type I,Col-I)、透明质酸酶(hyaluronidase-1,Hyal-1)、上皮性钙黏素(E-cadherin)、极性调控蛋白crumbs3(CRB3)的表达及分布情况.结果:HE染色结果提示,越靠近肿瘤病灶,大肠隐窝的破坏越严重.免疫组织化学实验结果表明,在1、2,3号位中Col-I和Hyal-1表达逐渐增高,E-cadherin和CRB3表达逐渐降低.结论:在大肠癌发生过程中,Col-I和Hyal-1表达增高,提示细胞外基质的改变可以促进肿瘤发生;而E-cadherin和CRB3表达降低提示细胞极性的丧失也是促进肿瘤发生的一个重要因素.AIM： To investigate the role of cancer microenvironment in colorectal carcinogenesis by detecting the expression of extracellular matrix and cell polarity proteins in tissues at different distances from colorectal cancer lesions. METHODS： Samples were collected from sites 10, 5, and 2 cm away from colorectal cancer lesions. HE staining was used to observe the structure of crypts. Immunohistochemistry was used to detect the expression of collagen type I （Col- I ）, hyaluronidase-1 （Hyal-1）, E-cadherin and crumbs3 （CRB3）. RESULTS： As the tissues were getting closer to the colorectal cancer lesions, the expression of Col- I and Hyal-1 increased, while that of E-cadherin and CRB3 decreased. CONCLUSION： Up-regulation of Col- I and Hyal-1 and down-regulation of E-cadherin and CRB3 may contribute to the initiation of colorectal cancer.

关 键 词：肿瘤微环境 大肠癌 免疫组织化学 

分 类 号：R735.34[医药卫生—肿瘤]