同种异体骨支架复合自体骨髓间充质干细胞修复下颌骨节段缺损动物模型的建立  

Animal Model of Reconstruction of Beagle Large Segment of Mandibular Defect with Allogenic Mandibular Scaffold and Autologous Mesenchymal Stem Cells

作　　者：刘昌奎[1] 谭新颖[1] 罗金超[1] 黄海涛[1] 胡敏[1] 

机构地区：[1]解放军301医院口腔科,北京100039

出　　处：《口腔医学研究》2015年第1期27-30,共4页Journal of Oral Science Research

基　　金：北京市自然科学基金(项目编号:7112124)

摘　　要：目的:建立同种复合自体骨髓间充质干细胞修复下颌骨节段性的动物模型,为研究同种异体骨支架复合自体骨髓间充质干细胞修复下颌骨节段性缺损效果及其机制打下基础。方法:24只1岁龄比格犬,拔出所有动物右侧下颌前磨牙和磨牙,伤口愈合后制造左侧下颌骨3mm节段性缺损(从颏孔后1mm到下颌角前),随机分两组,实验组用同种异体骨支架复合骨髓间充质干细胞修复重建,对照组仅用同种异体骨修复重建。术后4、12、24、48周处死动物(每组3只),取标本并行CT检查。结果:实验组和对照组所有动物同种异体骨均能与自体骨愈合,48周时实验组动物同种异体骨几乎全部被自体骨替代,但是新骨的大小较植入时同种异体骨支架变小。对照组中,新骨主要在同种异体骨与自体骨结合部形成,新骨大小与原植入时同种异体骨大小变化不大。结论:同种异体骨支架复合自体骨髓间充质干细胞能加速成骨,该动物模型的成功建立,为进一步研究同种异体骨支架复合自体骨髓间充质干细胞修复下颌骨节段行缺损的效果及其机制打下基础。Objective：To build a model of repairing large segmental of mandibular defect using engineering bone with allogenic mandibular scaffold and autologous mesenchymal stem cells.Methods：Twenty-four beagles,weighing 10 to 15kg,received 30 mm bone surgical defects in each left body of the mandible.The Beagles were divided into 2groups.The bony defect of the control group（12beagles）were reconstructed using allograft only.The experimental group（12beagles）were reconstructed using allogenic mandibular scaffold ioaded autologous mesenchymal stem cells.Beagles from each treatment were sacrificed at 4weeks,12 weeks,24weeks,or 48 weeks after surgery.Computed tomography were used to evaluate the reconstruction of the defect.Results：Results showed that the experimental group and the control group,allogeneic bone could heal with autologous bone,and could be gradually replaced by autogenous bone.A year later,the allogenic mandibular scaffold of the experimental group were completely replaced by new bone.But the size of the new bone was smaller than original allogenic scaffold.In the control group,the new bone was formed in both ends.The size of the allogenic scaffold wasn’t changed compared with original allogenic scaffold.Conclusion：The successful establishment of animal models provides basis for further study the worth and the mechanism of allogeneic bone scaffold combined with autologous bone marrow mesenchymal stem cells to repair segmental mandibular defect.

关 键 词：骨髓间充质干细胞 同种异体骨 下颌骨 修复重建 组织工程 

分 类 号：R-332[医药卫生] R782.4