磷酸三钙/壳聚糖支架与牙周膜干细胞混合培养的动物实验  

Animal Experiment of PDLSC Cultured on TCP/Chitosan Scaffold in Vivo

作　　者：钟志华[1] 施斌[2] 周先略[1] 

机构地区：[1]荆州市中心医院口腔科,湖北荆州434000 [2]武汉大学口腔医院种植科

出　　处：《口腔医学研究》2015年第1期31-33,共3页Journal of Oral Science Research

基　　金：湖北省卫生厅资助项目(2009-JX4B62)

摘　　要：目的:检测磷酸三钙(TCP)/壳聚糖支架在动物实验中是否适合培养牙周膜干细胞(PDLSC)。方法:制作3种不同比例TCP/壳聚糖支架,加入PDLSC和TCP/壳聚糖支架混合培养24h后植入裸鼠背部皮下,4周后切取移植物,HE染色和Ⅰ型胶原(Col-Ⅰ)免疫组化染色,镜检。结果:PDLSC在TCP/壳聚糖支架上生长良好,TCP/壳聚糖支架比单纯壳聚糖支架降解率减低,3组支架均有Col-Ⅰ生成。结论:TCP/壳聚糖支架适合培养PDLSC。Objective：To test TCP/Chitosan scaffold that PDLSC were cultuled.Methods：We prepared three kinds of TCP/Chitosan scaffolds,cultured PDLSC on TCP/Chitosan scaffold for 24 hrs,and then transplanted it subcutaneously into athymic mice.Implants were taken out after 4weeks and stained by HE and Col-Ⅰ.Then we observed them by lightscope.Results：PDLSC grow well on TCP/Chitosan scaffold.Degradation rate of TCP/Chitosan scaffold was lower than pure Chitosan scaffold.Col-Ⅰ was observed in three kinds of scaffolds.Conclusion：TCP/Chitosan scaffold is good for culturing PDLSC.

关 键 词：牙周膜干细胞 磷酸三钙 壳聚糖 Ⅰ型胶原 

分 类 号：R783.1[医药卫生—口腔医学]