Neutrophil transcriptional profile changes during transii from bone marrow to sites of inflammation  

作　　者：Flavia S Lakschevitz Michelle B Visser Chunxiang Sun Michael Glogauer 

机构地区：[1]Department of Periodontology, Faculty of Dentistry, University of Toronto, Toronto, Ont., Canada [2]Matrix Dynamics Group, Faculty of Dentistry, University of Toronto, Toronto, Ont., Canada [3]Current address: Department of Oral Biology, School of Dental Medicine, U niversity at Buffalo, The State University of New York, Buffalo, NY, USA

出　　处：《Cellular & Molecular Immunology》2015年第1期53-65,共13页中国免疫学杂志（英文版）

摘　　要：It has recently been established that neutrophils, the most abundant leukocytes, are capable of changes in gene expression during inflammatory responses. However, changes in the transcriptome as the neutrophil leaves the bone marrow have yet to be described. We hypothesized that neutrophils are transcriptionally active cells that alter their gene expression profiles as they migrate into the vasculature and then into inflamed tissues. Our goal was to provide an overview of how the neutrophil＇s transcriptome changes as they migrate through different compartments using microarray and bio-informatic approaches. Our study demonstrates that neutrophils are highly plastic cells where normal environmental cues result in a site-specific neutrophil transcriptome. We demonstrate that neutrophil genes undergo one of four distinct expression change patterns as they move from bone marrow through the circulation to sites of inflammation： （i） continuously increasing; （ii） continuously decreasing; （iii） a down-up-down; and （iv） an up-down-up pattern. Additionally, we demonstrate that the neutrophil migration signaling network and the balance between anti-apoptotic and pro-apoptotic signaling are two of the main regulatory mechanisms that change as the neutrophil transits through compartments.It has recently been established that neutrophils, the most abundant leukocytes, are capable of changes in gene expression during inflammatory responses. However, changes in the transcriptome as the neutrophil leaves the bone marrow have yet to be described. We hypothesized that neutrophils are transcriptionally active cells that alter their gene expression profiles as they migrate into the vasculature and then into inflamed tissues. Our goal was to provide an overview of how the neutrophil＇s transcriptome changes as they migrate through different compartments using microarray and bio-informatic approaches. Our study demonstrates that neutrophils are highly plastic cells where normal environmental cues result in a site-specific neutrophil transcriptome. We demonstrate that neutrophil genes undergo one of four distinct expression change patterns as they move from bone marrow through the circulation to sites of inflammation： （i） continuously increasing; （ii） continuously decreasing; （iii） a down-up-down; and （iv） an up-down-up pattern. Additionally, we demonstrate that the neutrophil migration signaling network and the balance between anti-apoptotic and pro-apoptotic signaling are two of the main regulatory mechanisms that change as the neutrophil transits through compartments.

关 键 词：fMLP signaling pathway MICROARRAY NEUTROPHIL TRANSCRIPTOME 

分 类 号：Q26[生物学—细胞生物学] X520.322.5[环境科学与工程—环境工程]