Temperature effect on translocation speed and capture rate of nanopore-based DNA detection  

作　　者：ZHANGYin WU GenSheng MA Jian YUAN ZhiShan SI Wei LIU Lei SHA JingJie CHEN YunFei 

机构地区：[1]Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, School of Mechanical Engineering, Southeast University

出　　处：《Science China(Technological Sciences)》2015年第3期519-525,共7页中国科学（技术科学英文版）

基　　金：supported by the National Basic Research Program of China("973"Project)(Grant Nos.2011CB707601 and 2011CB707605);the National Natural Science Foundation of China(Grants Nos.50925519 and 51375092);supported by the Scientific Research Foundation of Graduate School of Southeast University(Grant No.YBJJ1004);supported by the Fundamental Research Funds for the Central Universities and the Innovative Project for Graduate Students of Jiangsu Province(Grant No.KYLX_0100)

摘　　要：We study the effects of electrolyte temperature on DNA molecule translocation experimentally without and with a temperature gradient across nanopore membranes.The same temperatures on both electrolyte chambers are first considered.The DNA molecule translocation time is measured to be 2.44 ms at 2°C in both chambers,which is 1.57 times longer than at 20°C.Then the temperature difference effect is characterized in both chambers.The results show that the DNA translocation speed can be slowed down as long as one side temperature is lowered,irrespective of the temperature gradient direction.This indicates that the thermophoretic driving force generated by a temperature gradient has no obvious effect on the threading speed of DNA molecules,while the main reason for the slowed DNA translocation speed is the increased viscosity.Interestingly,the capture rate of DNA molecules is enhanced under a temperature gradient condition,and the capture rate during DNA translocation from hot side at 21°C to cold one at 2°C is 1.7 times larger than that under the condition of both chambers at 20°C.Finally,an optimized configuration is proposed to acquire higher capture rates and lower DNA translocation speeds.

关 键 词：NANOPORE DNA detection temperature translocation speed capture rate 

分 类 号：Q523[生物学—生物化学] TU375.1[建筑科学—结构工程]