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Nanopore靶向测序在呼吸道样本中检测非结核分枝杆菌的诊断价值研究: 《中国防痨杂志》2025年第5期589-596,共8页应广智蔡青山马晓卿陈凌燕陈园园; 国家自然科学基金(82104236)。; 目的:探讨Nanopore靶向测序在呼吸道样本中检测非结核分枝杆菌(non-tuberculous mycobacteria,NTM)的诊断价值。方法:回顾性收集2021年12月至2022年9月在浙江省杭州市红十字会医院结核病诊疗中心行呼吸道样本Nanopore靶向测序的455例患...; 关键词：非典型性细菌分枝杆菌感染 NANOPORES 诊断技术和方法敏感性与特异性

基于Nanopore技术的马铃薯甲虫全长转录组测序研究: 《植物检疫》2025年第1期1-7,共7页李红卫张柳李彤丁晓宇于艳雪; 中国检验检疫科学研究院基本科研业务费项目(2022JK09)。; 为深度挖掘马铃薯甲虫的转录组信息与功能基因,本研究采用Nanopore测序技术,针对马铃薯甲虫触角混合组织展开全长转录组测序,成功构建了该物种的全长转录本文库。在研究过程中,通过对转录组数据进行生物信息学分析,完成可变剪接分析、...; 关键词：马铃薯甲虫全长转录组转录因子简单重复序列长链非编码RNA 第三代测序技术纳米孔测序

Translocation of ssDNA through Charged Graphene Nanopores:Effect of the Charge Density: 《Chinese Journal of Polymer Science》2024年第12期2048-2058,I0013,共12页Yuan-Shuo Zhang Zhi-Ya Qi Ming-Ming Ding Ming-Lun Li Tong-Fei Shi; This work was financially supported by the National Natural Science Foundation of China(Nos.22373025 and 22227804).; Nanopore sequencing harnesses changes in ionic current as nucleotides traverse a nanopore,enabling real-time decoding of DNA/RNA sequences.The instruments for the dynamic behavior of substances in the nanopore on the ...; 关键词：Nanopore sequencing Graphene nanoslit DNA translocation Electroosmotic flow

Simultaneous detection of methylation and genetic variations of BCR-ABL1 gene by nanopore Cas9-targeted sequencing: 《Genes & Diseases》2024年第6期95-98,共4页Shuilian Xie Ying Yang Junjie Zhang Menglin Zhu Wang Li Mengting Li Yijian Chen Hailiang Li Weidan Lun Weelic Chong Shaogui Wan; The Youth Jinggang Scholars Program in Jiangxi Province of China,Jiangxi Natural Science Foundation (China) (No.20232BAB216017);the National Natural Science Foundation of China (No.82200653).; The BCR-ABL1 fusion gene is a driver and hallmark of leukemia and a classic structural variant.1 Single nucleotide variations(SNVs)occurring in the ABL1gene kinase domain(KD)and aberrant DNA methylation modifications,...; 关键词：TARGETED clinical THERAPEUTIC

Nanopore sequencing:flourishing in its teenage years: 《Journal of Genetics and Genomics》2024年第12期1361-1374,共14页Tianyuan Zhang Hanzhou Li Mian Jiang Huiyu Hou Yunyun Gao Yali Li Fuhao Wang Jun Wang Kai Peng Yong-Xin Liu; financially supported by the Natural Science Foundation of China(32470055 and U23A20148);the China Postdoctoral Science Foundation(2024M753580);the Agricultural Science and Technology Innovation Program(CAAS-ZDRW202308)。; Over the past decade,nanopore sequencing has experienced significant advancements and changes,transitioning from an initially emerging technology to a significant instrument in the field of genomic sequencing.However,...; 关键词：Nanopore sequencing Long-read sequencing Application Accuracy Outlook Direct RNA sequencing METAGENOMICS

Comparison of Ion Torrent,Illumina,and Nanopore platforms for sequencing SARS-CoV-2 JN.1 lineage variants from Dalian,China: 《感染控制(英文)》2024年第4期63-76,共14页Xingying Lang Yue Wang Mingchun Luan Zhijian Bo Huan Wang Xue Teng Xuehong Meng Shumin Niu Xiaoman Cui Nan Xia; supported by grants from the Dalian Science and Technology Innovation Fund(Grant No.2023JJ13SN040);the Dalian Medical Science Research Project(Grant No.2111033).; Objectives Dominant strains of SARS-CoV-2 have been replaced by SARS-CoV-2 JN.1 lineage variants,which have spread widely in a short period of time.However,an accurate detection method and the reason for rapid spread ...; 关键词：SARS-CoV-2 JN.1 lineage variation Ion Torrent sequencing Illumina sequencing Nanopore sequencing

Rapid diagnosis of Mycoplasma pneumoniae and prediction of antibiotic resistance by nanopore adaptive sampling: 《iLABMED》2024年第4期266-276,共11页Yunke Sun Xiaonan Li Jiale He Lingguo Zhao Qingliang Chen Lei Lei Jun Chen Lin Zhong Guobao Li Yu Xia Yanmin Bao Yingdan Zhang Liang Yang; Science,Technology and Innovation Commission of Shenzhen Municipality,Grant/Award Number:JCYJ20210324115607021;Medical Scientific Research Foundation of Guangdong Province of China,Grant/Award Number:A2021416;National Natural Science Foundation of China,Grant/Award Numbers:32200155,32270196;Shenzhen Science and Technology Program,Grant/Award Numbers:JCYJ20220530163412029,JCYJ20220531093409021,JCYJ20230807153604009;National Key Research and Development Program of China,Grant/Award Number:2022YFC2304700。; Background:Microbial infections,particularly in children,require rapid and accurate diagnostics.It is difficult to differentiate pathogens from commensal organisms,and it is impossible to identify antibiotic resistanc...; 关键词：antimicrobial resistance Mycoplasma pneumonia nanopore adaptive sampling

NanoTrans:an integrated computational framework for comprehensive transcriptome analysis with nanopore direct RNA sequencing: 《Journal of Genetics and Genomics》2024年第11期1300-1309,共10页Ludong Yang Xinxin Zhang Fan Wang Li Zhang Jing Li Jia-Xing Yue; facility support from the Single-Molecule Sequencing Platform at Sun Yat-sen University Cancer Center.This work is supported by the National Natural Science Foundation of China(32070592 to JXY,32000395 to JL,82272789 to LZ);Guangdong Basic and Applied Basic Research Foundation(2022A1515010717 and 2019A1515110762 to JXY and 2022A1515011873 to JL);Guangdong Pearl River Talents Program(2019QN01Y183 to JXY,2021QN02Y168 to JL);Guangzhou Municipal Science and Technology Bureau(202102020938 to JL);Young Talents Program of Sun Yat-sen University Cancer Center(YTP-SYSUCC-0042 to JXY and YTP-SYSUCC-0040 to JL)。; Nanopore direct RNA sequencing(DRS)provides the direct access to native RNA strands with full-length information,shedding light on rich qualitative and quantitative properties of gene expression profiles.Here with Nan...; 关键词：Direct RNA sequencing NANOPORE TRANSCRIPTOME Long reads DRS

Bioinspired polydopamine coated nanopore nanofluidic unijunction transistor exhibiting negative differential resistance and ion current oscillation: 《Nano Research》2024年第11期10026-10033,共8页Yong Wang Wenting Guo Bo Wang Ya Zhou Ping Hu Jiangtao Ren Erkang Wang Yongdong Jin; supported by the National Natural Science Foundation of China(Nos.22374145 and 21675146);the Jilin Province Science Technology Development Plan Project(No.20230508075RC);the Youth Innovation Promotion Association CAS(No.2021224).; Nanofluidic devices have turned out to be exemplary systems for investigating fluidic transport properties in a highly restricted area, where the electrostatic interactions or chemical reactions between nanochannel an...; 关键词：POLYDOPAMINE NANOPORE unijunction transistor negative differential resistance ion current oscillation

Nanopore ultra-long sequencing and adaptive sampling spur plant complete telomere-totelomere genome assembly: 《Molecular Plant》2024年第11期1773-1786,共14页Dongdong Lu Caijuan Liu Wenjun Ji Ruiyan Xia Shanshan Li Yanxia Liu Naixu Liu Yongqi Liu Xing Wang Deng Bosheng Li; supported by the Key R&D Program of Shandong Province,China(grant no.ZR202211070163);the National Natural Science Foundation of China(grant nos.32170574 and 32200249);the Natural Science Foundation of Shandong Province(grant nos.ZR2023QC026 and ZR2023QC106);the Young Taishan Scholars Program and Yuandu Scholars Program.; The pursuit of complete telomere-to-telomere(T2T)genome assembly in plants,challenged by genomic complexity,has been advanced by Oxford Nanopore Technologies(ONT),which offers ultra-long,realtime sequencing.Despite it...; 关键词：complete T2T genome ultra-long sequencing nanopore sequencing adaptive sampling filling gaps

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