Photosensitive damage of lysozyme caused by pazufloxacin and the protective effect of ferulic acid  

作　　者：Yulie Xu Yancheng Liu Haixia Li Peng Zhang Jianfeng Zhao Wenfeng Wang 

机构地区：[1]Shanghai Institute of Applied Physics, Chinese Academy of Sciences [2]University of Chinese Academy of Sciences

出　　处：《Science China Chemistry》2015年第3期508-513,共6页中国科学（化学英文版）

基　　金：supported by the National Natural Science Foundation of China(21173252)

摘　　要：Laser flash photolysis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE) experiments were carried out to study the photosensitive damage induced by pazufloxacin(PAX) and the protection of lysozyme(Lyso) by ferulic acid(FCA), an active antioxidant. Transient absorption spectra revealed electron transfer from Lyso and FCA to 3PAX*, with respective bimolecular reaction rate constants of 6.3×107 and 1.4×1010 dm3/(mol s). A pulse radiolysis study was also performed to investigate the formation of Lyso radical cations. Results showed that FCA effectively inhibited the cross-linking of protein induced by fluoroquinolones. Finally, a mechanism of the protective effect of FCA on Lyso was proposed.Laser flash photolysis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis（SDS-PAGE） experiments were carried out to study the photosensitive damage induced by pazufloxacin（PAX） and the protection of lysozyme（Lyso） by ferulic acid（FCA）, an active antioxidant. Transient absorption spectra revealed electron transfer from Lyso and FCA to ^3PAX^＊, with respective bimolecular reaction rate constants of 6.3×10^7 and 1.4×10^10 dm^3/（mol s）. A pulse radiolysis study was also performed to investigate the formation of Lyso radical cations. Results showed that FCA effectively inhibited the cross-linking of protein induced by fluoroquinolones. Finally, a mechanism of the protective effect of FCA on Lyso was proposed.

关 键 词：LYSOZYME PAZUFLOXACIN ferulic acid laser flash photolysis pulse radiolysis SDS-PAGE 

分 类 号：TQ460.1[化学工程—制药化工]