脐带血细胞因子诱导杀伤细胞对人膀胱移行癌细胞体外杀伤效应的实验研究  

Experimental efficacy of cord blood cytokine-induced kill cells on the anti-tumor effects against bladder transitional cell

作　　者：田峰[1] 唐来坤[1] 王春慧 

机构地区：[1]上海市第八人民医院泌尿科,200235 [2]上海赛傲生物技术有限公司

出　　处：《中国综合临床》2015年第3期200-202,共3页Clinical Medicine of China

基　　金：上海市卫生局青年科研项目（20114Y131）

摘　　要：目的研究脐带血来源的细胞因子诱导的杀伤细胞（CIK）体外对人膀胱移行癌细胞株的杀伤作用。方法采集脐带血单个核细胞，以白细胞介素2（IL-2）、γ干扰素（IFN-γ）、CD3Mc-Ab诱导制备CIK细胞，经流式细胞仪检测CIK细胞的表型，在体外以CCK-8方法测定脐血CIK细胞对T24细胞的杀伤效应。结果脐血CIK细胞对T24的杀瘤率在效靶比为1：2．5、1：5、1：10及1：20时可分别达到（18．98±2．61）％、（35．59±4．74）％、（45．34±5．03）％、（69．24±5．19）％。结论脐带血CIK细胞对人膀胱移行癌细胞有很强的杀伤活性，对临床使用脐带血CIK细胞治疗膀胱癌有一定指导意义。Objective To investigate the effect of cytokine-induced kill cells （CIK） of the cord blood on bladder transitional cell carcinoma T24 in vitro. Methods The cord blood mononuclear cells （CBMC） were induced with different cytokines such as Interleukin 2 （IL-2）, interferon γ （IFN-γ）, CD3Mc-Ab into CIK cells. The proliferation ability of CIK cells was measured by calculation in different culturing time, and the phenotype of CIK cells was analyzed by the flow cytometry. Bladder transitional cell carcinoma T24 cells were mixed with CIK cells to detect the mortality of the cancer cells with CCK-8 method in vitro. Results With the cultured time of CIK ceils prolong, both the number and killing activity increased, the ratio of CIK cells and T24 cells were 1：2. 5,1：5,1 ： 10 and 1：20 when the kill rate of T24 cells by CIK cells were （ 18.98 ± 2. 61 ） % , （35. 59 ±4. 74）% ,（45.34 ±5.03）% and （69. 24 ±5. 19）%. Conclusion The CIK cells of the cord blood is proved to be with strong anti-cancer activity against T24 cells in vitro.

关 键 词：脐带血细胞因子诱导杀伤细胞 细胞培养 T24 

分 类 号：R737.25[医药卫生—肿瘤]