TGF-β1、Smad7与树突状细胞在结直肠癌肝转移中作用的初步研究  

Preliminary study of roles of TGF-β1, Smad7 and dendritic cells in colorectal cancer liver metastases

作　　者：刘贤伟[1] 曹俊达 熊功友[1] 余昌达 张建华[3] 董钦生[3] 周丁华[3] 

机构地区：[1]江西省九江市第一人民医院普通外科,北京100088 [2]江西省九江市第一人民医院心血管内科,江西九江332200 [3]中国人民解放军第二炮兵总医院普通外科,北京100088

出　　处：《中国普通外科杂志》2015年第1期63-69,共7页China Journal of General Surgery

摘　　要：目的:探讨结直肠癌肝转移(CRLM)组织中TGF-β1、Smad7表达和树突状细胞(DC)的变化及临床意义。方法:采用免疫组化法检测TGF-β1、Smad7、CD1α(未成熟DC标志)和CD83(成熟DC标志)在CRLM组织和CRLM术后切缘阴性的正常肝组织中的表达,分析CRLM组织中以上指标表达之间的关系以及与原发灶特征的关系。结果:CRLM组织中TGF-β1、Smad7、和CD1α的表达明显高于正常肝组织组(均P<0.05),且CRLM组织中彼此表达之间呈正相关关系(r=0.455、r=0.623、r=0.448,均P<0.05);CD83在CRLM组织中基本为全阴性表达,而在正常肝组织组中仅2例呈弱阳性表达。TGF-β1、Smad7、和CD1α的表达均与CRLM患者原发灶的肿瘤浸润深度、有无淋巴结转移有关(均P<0.05),而与原发灶肿瘤分化程度无关(P>0.05)。结论:TGF-β1、Smad7α的高表达及未成熟DC数量的增加可能共同促进CRLM的发生、发展。Objective： To investigate the expressions of TGF-β1 and Smad7 and change of dendritic cells（DCs） in colorectal cancer liver metastases（CRLM） tissue and the clinical significance.Methods： The expressions of TGF-β1, Smad7, CD1α（marker of immature DCs） and CD83（marker of mature DCs） in resection specimens of CRLM tissue and normal liver tissue around the CRLM with negative margin were determined by immunohistochemical staining. The mutual relations among these expressions in CRLM tissue and their relations with the characteristics of the primary tumor were analyzed.Results： The expressions of TGF-β1, Smad7, and CD1α in CRLM tissue were significantly higher than those innormal liver tissue（all P0.05）, and their expressions showed mutual positive correlations in CRLM tissue（r=0.455, r=0.623, r=0.448, all P0.05）; CD83 expression was negative in almost all the studied CRLM tissue specimens, and only two cases of weak positive expression were found in the normal liver tissue specimens. All expressions of TGF-β1, Smad7 and CD1α were significantly related to the depth of invasion of the primary tumor and lymph node metastasis（all P0.05）, but irrelevant to dif erentiation of the primary tumor（P0.05） in CRLM patients.Conclusion： The high TGF-β1 and Smad7 expressions and increased immature DCs number may together promote the occurrence and development of CRLM.

关 键 词：肝肿瘤/继发性 结直肠肿瘤 肿瘤转移 树突状细胞 转化生长因子Β1 

分 类 号：R735.7[医药卫生—肿瘤]