VEGFR-3 siRNA腺病毒表达载体对结肠癌细胞凋亡和侵袭的影响  

Effects of the adenovirus expressing the small interfering RNA targeting vascular endothelia growth factor receptor-3 gene on apoptosis and invasion of human colon cancer cells

作　　者：吕志诚[1] 苏芝兰 马强[3] 张鑫[1] 

机构地区：[1]兰州军区兰州总医院肛肠外科,甘肃兰州730050 [2]甘肃省武威市医院消化科 [3]兰州军区兰州总医院消化科

出　　处：《胃肠病学和肝病学杂志》2015年第1期18-21,共4页Chinese Journal of Gastroenterology and Hepatology

基　　金：甘肃省对外合作基金资助项目(1104WCGA190)

摘　　要：目的探讨靶向血管内皮细胞生长因子受体3(vascular endothelial growth factor receptor-3,VEGFR-3)基因的小干扰RNA(small interfering RNA,siRNA)腺病毒载体对人结肠癌Lo Vo细胞系凋亡及侵袭的影响。方法将靶向VEGFR-3 siRNA腺病毒转染结肠癌Lo Vo细胞,以Western blotting检测VEGFR-3蛋白的表达,Hoechst 33342染色法和流式细胞仪检测Lo Vo细胞的凋亡情况,用Transwell小室测定Lo Vo细胞的侵袭力。结果实验组与空白对照组和阴性对照组比较,实验组中转染靶向VEGFR-3 siRNA腺病毒后结肠癌Lo Vo细胞中VEGFR-3蛋白的表达被下调(P<0.05)。Hoechst 33342染色法和流式细胞仪检测Lo Vo细胞凋亡率明显升高(P<0.05),Transwell小室测定Lo Vo细胞侵袭能力下降(P<0.05)。结论靶向VEGFR-3 siRNA腺病毒能下调结肠癌LoVo细胞中VEGFR-3蛋白的表达,从而诱导结肠癌Lo Vo细胞的凋亡,抑制其侵袭能力,因此,VEGFR-3可以作为结肠癌靶向治疗的一个潜在靶点。Objective To investigate the effects of the adenovirus expression vector of small interfering RNA （ siR-NA） targeting vascular endothelia growth factor receptor 3 （VEGFR-3） gene on apoptosis and invasion of human colon cancer LoVo cells. Methods VEGFR-3 siRNA adenovirus was transfected into the LoVo cells, and the expressions of VEGFR-3 were measured by Western blotting. The apoptosis was detected by Hoechst 33342 staining and FCM （ flow cytometry） . The invasion ability was assayed by Transwell method. Results Compared with the blank control group and the negative control group, the expression level of VEGFR-3 protein in LoVo cells of experimental group was re-duced after transfection with pAd-VEGFR3-siRNA （ P 〈0 . 05 ） . The apoptosis rate was increased significantly （ P 〈0. 05）. The proliferation of LoVo cells was inhibited, the invasion ability decreased sinificantly （P〈0. 05）. Conclu-sion VEGFR-3 siRNA can down-regulate the expression levels of VEGFR-3 protein in LoVo cells, inhibit the invasion abilities of LoVo cells, and induce the apoptosis of LoVo cells effectively. VEGFR-3 can be used as a potential aim of colon cancer targeted therapy.

关 键 词：结肠肿瘤 RNA干扰 血管内皮细胞生长因子受体3 基因治疗 腺病毒 侵袭 凋亡 

分 类 号：R735.35[医药卫生—肿瘤]