槲皮素对刀豆蛋白A诱导小鼠急性肝损伤的保护作用  

Protective effects of quercetin on Con A-induced acute liver injury in mice

作　　者：翁书强[1] 姚群燕[1] 李希[1] 涂传涛[1] 沈锡中[1] 

机构地区：[1]复旦大学附属中山医院消化科,上海200032

出　　处：《胃肠病学和肝病学杂志》2015年第1期40-44,共5页Chinese Journal of Gastroenterology and Hepatology

摘　　要：目的探讨槲皮素对刀豆蛋白A(Concanavalin A,Con A)诱导小鼠急性肝损伤的保护作用及其可能的分子机制。方法通过检测Con A注射后8 h血清转氨酶水平及肝组织病理学评估肝损伤;应用酶联免疫法(ELISA)检测TNF-α、IFN-γ、IL-4水平;定量PCR和Western blotting或免疫组织化学检测肝组织mRNA与蛋白的表达。结果与模型组相比,槲皮素预防组显著降低了血清转氨酶水平(P<0.05),且组织病理学分析表明肝脏炎症坏死程度明显减轻;与模型组相比,槲皮素预防组显著降低了血清促炎症细胞因子TNF-α、IFN-γ和IL-4的水平(P<0.01);槲皮素预防组肝组织NOX4、COX-2和i NOS基因与蛋白的表达均明显低于模型组(P<0.05)。结论槲皮素对Con A诱导小鼠急性肝损伤具有保护作用,其发挥有益作用的机制可能与抑制肝脏的炎症反应有关。Objective To investigate the hepatoprotective effect and the potential molecular mechanisms of quercetin on Concanavalin A （ ConA）-induced acute liver injury in mice. Methods The liver injury was examined by measuring serum transaminase 8 h after ConA injection and liver histology. The tumor necrosis factor-α （ TNF-α） , interferon-γ（ IFN-γ） , and interleukin-4 （ IL-4 ） levels in liver tissue were detected by enzyme linked immunosorbent assay （ELISA）. Furthermore, the gene and protein expression of NADPH oxidase 4 （NOX4）, cyclooxygenase-2 （COX-2） and inducible nitric oxide synthase （ iNOS） in liver were detected by Real-time quantitative PCR and Western blotting or immunohistochemical staining, respectively. Results Quercetin significantly decreased the elevated plasma levels of aminotransferases compared with model group （ P〈0 . 05 ） . Histopathological analysis also showed that quercetin preven-ted inflammation and necrosis. Also, quercetin pretreatment reduced the serum levels of pro-inflammatory molecules such as TNF-α, IFN-γ and IL-4 compared with the vehicle controls （P〈0. 05）. Furthermore, the expression levels of NOX4, COX-2 and iNOS mRNA or protein in liver tissues were significantly lowered by quercetin pretreatment. Conclu-sion Quercetin pretreatment protects against ConA-induced acute liver injury in mice, and the beneficial effect of quer-cetin may be related to inhibition of the inflammatory response in liver.

关 键 词：槲皮素 刀豆蛋白A 急性肝损伤 NADPH氧化酶4 环氧合酶-2 可诱导型一氧化氮合酶 

分 类 号：R575[医药卫生—消化系统]