EZH2及DNMT1在三阴性乳腺癌中的表达及意义  

Expression and significance of EZH2 and DNMT1 in triple-negative breast cancer

作　　者：武萍萍[1] 陶然[2] 彭攸[3] 赵卫卫[3] 刘萃萃[3] 王璐璐[3] 胡朝晖[2] 王玉平[3] 孙振亮[3] 冯景[3] 

机构地区：[1]南方医科大学第三临床医学院,广州510515 [2]广州金域医学检验中心有限公司,广州510330 [3]南方医科大学附属奉贤医院检验科,上海201400

出　　处：《解放军医学杂志》2015年第1期50-55,共6页Medical Journal of Chinese People's Liberation Army

基　　金：上海市自然基金项目(12ZR1426300);上海市卫生系统优秀学科带头人培养计划(XBR20130114)~~

摘　　要：目的探讨EZH2及DNMT1在三阴性乳腺癌中的表达特点及与主要临床病理参数的关系。方法采用免疫组化SP法,检测131例三阴性乳腺癌、78例非三阴性乳腺癌及65例癌旁组织中EZH2及DNMT1的表达情况。采用多因素logistic回归分析EZH2及DNMT1阳性表达有关的因素,Spearman等级相关分析三阴性乳腺癌中EZH2及DNMT1表达的相关关系。结果 EZH2蛋白在三阴性乳腺癌组织、非三阴性乳腺癌组织和癌旁组织的阳性表达率分别为86.3%、89.7%和40.0%;DNMT1蛋白在三种组织中的阳性表达率分别为63.4%、66.6%和44.6%;两种蛋白在乳腺癌组织中的表达均高于癌旁组织(P<0.05);两种蛋白在三阴性乳腺癌与非三阴性乳腺癌组织间表达差异无统计学意义。在三阴性乳腺癌中,EZH2阳性表达与肿瘤组织学分级、肿瘤直径、淋巴结是否转移有关(P<0.05),DNMT1的表达与肿瘤组织学分级、淋巴结是否转移有关(P<0.05)。多因素logistic回归分析显示,肿瘤组织学分级为影响EZH2及DNMT1表达的最主要因素;三阴性乳腺癌中DNMT1与EZH2的表达呈正相关(r=0.34,P<0.01)。结论 EZH2与DNMT1的高表达与三阴性乳腺癌的分化、生长、转移有关,且两者在三阴性乳腺癌中的表达呈正相关,其联合检测可能对三阴性乳腺癌的预后判断更有价值。Objective To probe into the expression features of EZH2 and DNMT1 in triple-negative breast cancer （TNBC）, and the relations between the expression and the main clinical pathological parameters of TNBC. Methods The clinical and pathological data of 209 cases of breast cancer （including 131 cases of TNBC and 78 cases of non-TNBC） in Guangzhou Kingmed Center for Clinical Laboratory from Jan. 2008 to May 2013 were retrospectively analyzed. Immunohistochemistry SP assay was performed to determine the expressions of EZH2 and DNMT1 in 209 paraffin-embedded specimens of breast cancer and 65 specimens of normal tissue （more than 4cm away from tumor）. The differences of protein expression in the specimens were compared, and correlation analysis was performed to disclose the relationship between protein expression and clinico-pathological indices. Multivariate logistic regression analysis was applied to analyze the positive expression-related factor of EZH2 and DNMT 1. Spearman rank correlation analysis was used to analyse the interrelation of EZH2 and DNMT1 in TNBC. Results The positive expression rates of EZH2 in TNBC, non-TNBC and adjacent breast tissue were 86.3%, 89.7% and 40.0%, respectively, while the positive expression rates of DNMT 1 were 63.4%, 66.6% and 44.6%, respectively. The positive expression rates of EZH2 and DNMT 1 were higher in both TNBC and non-TNBC tissues than in adjacent breast tissue （P〈0.05）, but no significant difference in expression rate between the TNBC tissues and non-TNBC tissues. There was no relationship between EZH2 ex0ression and are, menstrual status, histologic type and vessel and nerve invasion （P〉0.05）, but the positive expression of EZH2 in TNBC was related to the pathological grade, tumor size and lymph node metastasis （P〈0.05）） and the positive expression of DNMT1 was related to the pathological grade and lymph node metastasis （P〈0.05）. Multivariate logistic regression analysis demonstrated that the pathological grade of tumor was the main fa

关 键 词：乳腺肿瘤 组蛋白甲基转移酶同源序列2增强子 DNA甲基转移酶1 免疫组织化学 

分 类 号：R737.9[医药卫生—肿瘤]